Construct: ORF TRCN0000491647
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011668.1_s317c1
- Derived from:
- ccsbBroadEn_13384
- DNA Barcode:
- TCATACCTCGTAAATGCCTGCGTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SLC51A (200931)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491647
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 200931 | SLC51A | solute carrier family 51 al... | NM_152672.6 | 60.6% | 60.5% | 1_399del;604G>A;675T>C |
| 2 | mouse | 106407 | Slc51a | solute carrier family 51, a... | NM_145932.3 | 51.3% | 50.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 687
- ORF length:
- 621
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggt ggaaggcttt ggggggaagg aggcagtgct gaggacgctg agggacaccc 121 cgatgatggt ccacacaggc ccctgctgct gctgctgccc ctgctgtcca cggctgctgc 181 tcaccaggaa gaagcttcag ctgctgatgt tgggcccttt ccaatacgcc ttcttgaaga 241 taacgctgac cctggtgggc ctgtttctca tccccgacgg catctatgac ccagcagaca 301 tttctgaggg GAGCACAGCT CTATGGATCA ACACTTTCCT CGGCGTGTCC ACACTGCTGG 361 CTCTCTGGAC CCTGGGCATC ATTTCCCGTC AAGCCAGGCT ACACCTGGGT GAGCAGAACA 421 TGGGAGCCAA ATTTGCTCTG TTCCAGGTTC TCCTCATCCT GACTGCCCTA CAGCCCTCCA 481 TCTTCTCAGT CTTGGCCAAC GGTGGGCAGA TTGCTTGTTC GCCTCCCTAT TCCTCTAAAA 541 CCAGGTCTCA AGTGATGAAT TGCCACCTCC TCATACTGGA GACTTTTCTA ATGACTGTGC 601 TGACACGAAT GTACTACCGA AGGAAAGACC ACAAGGTTGG GTATGAAACT TTCTCTTCTC 661 CAGACCTGGA CTTGAACCTC AAAGCCTACC CAACTTTCTT GTACAAAGTG GTTGATATCG 721 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 781 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GATCATACCT 841 CGTAAATGCC TGCGTTACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 901 aagatt