Construct: ORF TRCN0000491680
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004926.1_s317c1
- Derived from:
- ccsbBroadEn_15992
- DNA Barcode:
- GTCTGCCGGCGAAAACCTATATGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- VASH2 (79805)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491680
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 79805 | VASH2 | vasohibin 2 | NM_001136475.3 | 61.7% | 60.9% | (many diffs) |
| 2 | human | 79805 | VASH2 | vasohibin 2 | NM_001136474.3 | 53.4% | 52.7% | (many diffs) |
| 3 | human | 79805 | VASH2 | vasohibin 2 | NM_024749.5 | 47.7% | 42.4% | (many diffs) |
| 4 | human | 79805 | VASH2 | vasohibin 2 | NM_001301056.2 | 43.6% | 43% | (many diffs) |
| 5 | mouse | 226841 | Vash2 | vasohibin 2 | NM_144879.2 | 39.3% | 42.5% | (many diffs) |
| 6 | mouse | 226841 | Vash2 | vasohibin 2 | XM_006497149.4 | 35.3% | 38.2% | (many diffs) |
| 7 | mouse | 226841 | Vash2 | vasohibin 2 | XM_006497150.4 | 35.3% | 38.2% | (many diffs) |
| 8 | mouse | 226841 | Vash2 | vasohibin 2 | XM_011238913.3 | 35.3% | 38.2% | (many diffs) |
| 9 | mouse | 226841 | Vash2 | vasohibin 2 | NR_027352.1 | 10.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 534
- ORF length:
- 468
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga aacagcaaaa gaaatgaccc gagagtcctt gcctatcaaa tgccttgaag 121 ctgtcatcct gggcatctac ttaaccaatg ggcagccttc cattgagcgg ttccccatca 181 gctttaaaac cTACTTCTCA GGAAACTACT TTCACCACGT TGTGCTGGGG ATTTACTGCA 241 ATGGCCGCTA TGGCTCATTG GGCATGAGCC GCAGGGCTGA GCTGATGGAC AAGCCATTGA 301 CTTTTCGGAC TCTGAGTGAC CTCATCTTTG ACTTTGAGGA CTCTTACAAG AAATACCTGC 361 ACACAGTCAA GAAGGTCAAG ATTGGGCTGT ACGTCCCCCA TGAGCCTCAT AGCTTCCAGC 421 CCATTGAGTG GAAGCAGCTG GTCCTCAACG TCTCAAAGAT GCTGAGGGCT GACATAAGGA 481 AGGAGCTGGA GAAATATGCC AGGGACATGA GAATGAAGGG CTTGTGCAGT CACGACCCAA 541 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 601 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 661 TATCTTGTGG AAAGGACGAG TCTGCCGGCG AAAACCTATA TGCACGCGTT AAGTCgacaa 721 tcaacctctg gattacaaaa tttgtgaaag att