Construct: ORF TRCN0000491702
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004544.1_s317c1
- Derived from:
- ccsbBroadEn_08141
- DNA Barcode:
- ATATTGACTTGAATGGCATATTCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ALG6 (29929)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491702
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 29929 | ALG6 | ALG6 alpha-1,3-glucosyltran... | NM_013339.4 | 99.9% | 99.8% | 911C>T |
| 2 | mouse | 320438 | Alg6 | asparagine-linked glycosyla... | NM_001081264.1 | 85.6% | 84.6% | (many diffs) |
| 3 | mouse | 320438 | Alg6 | asparagine-linked glycosyla... | XM_006503155.1 | 80.2% | 78.8% | (many diffs) |
| 4 | mouse | 320438 | Alg6 | asparagine-linked glycosyla... | XM_006503156.1 | 69.2% | 64.2% | (many diffs) |
| 5 | mouse | 320438 | Alg6 | asparagine-linked glycosyla... | XM_006503158.2 | 66.9% | 66% | (many diffs) |
| 6 | mouse | 320438 | Alg6 | asparagine-linked glycosyla... | XM_006503160.1 | 63.7% | 59.7% | (many diffs) |
| 7 | mouse | 320438 | Alg6 | asparagine-linked glycosyla... | XM_030253605.1 | 63.7% | 59.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1587
- ORF length:
- 1521
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga gaaatggtac ttgatgacag tagtggtttt aataggacta acagtacgat 121 ggacagtgtc tcttaattct tattcaggtg ctggtaaacc gcctatgttt ggtgattatg 181 aagctcagag acactggcaa gaaataactt ttaatttacc ggtcaaacaa tggtatttta 241 acagcagtga taacaattta cagtattggg gattggatta cccacctctt acagcttatc 301 atagtctcct atgtgcatat gtggcaaagt ttataaatcc agactggatt gctctccata 361 catcacgtgg atatgagagt caggcacata agctcttcat gcgtacaaca gttttaattg 421 ctgatctgct gatttacata cctgcagtgg ttttgtactg ttgttgctta aaagaaatct 481 caactaagaa aaagattgct aatgcattat gcatcttgct gtatccaggc cttattctta 541 tagactatgg acattttcaa tataattctg tgagtcttgg ctttgctttg tggggtgttc 601 ttggaatatc ttgtgactgc gacctcctag ggtcactggc attttgctta gctataaatt 661 ataaacagat ggaactttac cacgccttgc catttttttg ctttttactt ggcaagtgtt 721 ttaaaaaagg cctcaaagga aaggggtttg tgttgctagt taagctagct tgtattgttg 781 tggcttcctt cgttctctgc tggctgccat tctttacaga aagggaacaa accctgcagg 841 ttctaagaag actcttcccg gttgatcgtg gattatttga ggataaagta gccaatattt 901 ggtgcagctt caatgtcttt ctgaagatta aggatatttt gccacgtcac atccaattaa 961 taatgagctt ttgttttacg tttttgagcc tgcttcctgc atgcataaaa ttaatacttc 1021 agccctcttc caaaggattc aaatttacac tggttagctg tgcgctatca ttctttttat 1081 tttctttcca agtacatgaa aaatccattc tcttggtgtc actaccagtc tgcttagttt 1141 taagtgaaat tccttttatg tctacttggt ttttacttgt gtcaacattt agtatgctac 1201 ctcttctatt gaaggatgaa ctcctaatgc cctctgttgt gacaacaatg gcatttttta 1261 tagcttgtgt aacttccttt tcaatatttg aaaagacttc TGAAGAAGAA CTGCAGTTGA 1321 AATCCTTTTC CATTTCTGTG AGGAAATATC TTCCATGTTT TACATTTCTT TCCAGAATTA 1381 TACAATATTT GTTTCTTATC TCAGTCATCA CTATGGTGCT TCTGACGTTG ATGACTGTCA 1441 CACTGGATCC TCCTCAGAAA CTACCGGACT TGTTTTCTGT ATTGGTGTGT TTTGTATCTT 1501 GCTTGAACTT CCTGTTCTTC TTGGTATACT TTAACATTAT TATTATGTGG GATTCCAAAA 1561 GTGGAAGAAA TCAGAAGAAA ATCAGCTACC CAACTTTCTT GTACAAAGTG GTTGATATCG 1621 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 1681 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GAATATTGAC 1741 TTGAATGGCA TATTCAACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 1801 aagatt