Construct: ORF TRCN0000491741

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF020288.2_s317c1
DNA Barcode:
TACGCAACGCCATGAATTACAAAA
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Has stop codon in insert

Originally Annotated References:

Gene:
TPK1 (27010)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000491741
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 27010 TPK1 thiamin pyrophosphokinase 1 NM_001350885.1 99.2% 99.2% 411_412insTTG
2 human 27010 TPK1 thiamin pyrophosphokinase 1 NM_001350886.1 99.2% 99.2% 411_412insTTG
3 human 27010 TPK1 thiamin pyrophosphokinase 1 NM_001350887.1 99.2% 99.2% 411_412insTTG
4 human 27010 TPK1 thiamin pyrophosphokinase 1 NM_001350889.1 99.2% 99.2% 411_412insTTG
5 human 27010 TPK1 thiamin pyrophosphokinase 1 NM_001350893.1 99.2% 99.2% 411_412insTTG
6 human 27010 TPK1 thiamin pyrophosphokinase 1 NM_001350894.1 99.2% 99.2% 411_412insTTG
7 human 27010 TPK1 thiamin pyrophosphokinase 1 XM_011516047.2 99.2% 99.2% 411_412insTTG
8 human 27010 TPK1 thiamin pyrophosphokinase 1 XM_011516048.1 99.2% 99.2% 411_412insTTG
9 human 27010 TPK1 thiamin pyrophosphokinase 1 XM_017011982.1 99.2% 99.2% 411_412insTTG
10 human 27010 TPK1 thiamin pyrophosphokinase 1 XM_024446717.1 99.2% 99.2% 411_412insTTG
11 human 27010 TPK1 thiamin pyrophosphokinase 1 NM_001350895.1 91% 90.5% 0_1ins33;2T>A;378_379insTTG
12 human 27010 TPK1 thiamin pyrophosphokinase 1 XM_024446716.1 91% 90.5% 0_1ins33;2T>A;378_379insTTG
13 human 27010 TPK1 thiamin pyrophosphokinase 1 XM_017011980.2 83.5% 83.5% 1_78del;489_490insTTG
14 human 27010 TPK1 thiamin pyrophosphokinase 1 XM_017011981.2 83.5% 83.5% 1_78del;489_490insTTG
15 human 27010 TPK1 thiamin pyrophosphokinase 1 XM_024446715.1 60.4% 36.8% (many diffs)
16 human 27010 TPK1 thiamin pyrophosphokinase 1 NM_001350882.1 57.3% 57.3% 1_303del;714_715insTTG
17 human 27010 TPK1 thiamin pyrophosphokinase 1 NM_001350883.1 57.3% 57.3% 1_303del;714_715insTTG
18 human 27010 TPK1 thiamin pyrophosphokinase 1 NM_001350884.2 57.3% 57.3% 1_303del;714_715insTTG
19 human 27010 TPK1 thiamin pyrophosphokinase 1 NM_001350879.1 56.1% 56.1% 1_318del;729_730insTTG
20 human 27010 TPK1 thiamin pyrophosphokinase 1 NM_022445.4 56.1% 56.1% 1_318del;729_730insTTG
21 human 27010 TPK1 thiamin pyrophosphokinase 1 XM_005249970.1 56.1% 56.1% 1_318del;729_730insTTG
22 human 27010 TPK1 thiamin pyrophosphokinase 1 XM_011516039.2 56.1% 56.1% 1_318del;729_730insTTG
23 human 27010 TPK1 thiamin pyrophosphokinase 1 XM_017011974.1 56.1% 56.1% 1_318del;729_730insTTG
24 human 27010 TPK1 thiamin pyrophosphokinase 1 XM_017011975.1 56.1% 56.1% 1_318del;729_730insTTG
25 human 27010 TPK1 thiamin pyrophosphokinase 1 NM_001042482.1 55.3% 36% (many diffs)
26 human 27010 TPK1 thiamin pyrophosphokinase 1 NM_001350880.1 55.3% 36% (many diffs)
27 human 27010 TPK1 thiamin pyrophosphokinase 1 XM_011516037.2 51.6% 51.6% 1_381del;792_793insTTG
28 human 27010 TPK1 thiamin pyrophosphokinase 1 XM_017011970.1 51.6% 51.6% 1_381del;792_793insTTG
29 human 27010 TPK1 thiamin pyrophosphokinase 1 XM_017011971.1 51.6% 51.6% 1_381del;792_793insTTG
30 human 27010 TPK1 thiamin pyrophosphokinase 1 XM_011516031.1 50.7% 50.7% 1_396del;807_808insTTG
31 human 27010 TPK1 thiamin pyrophosphokinase 1 XM_011516032.2 50.7% 50.7% 1_396del;807_808insTTG
32 human 27010 TPK1 thiamin pyrophosphokinase 1 XM_011516033.2 50.7% 50.7% 1_396del;807_808insTTG
33 human 27010 TPK1 thiamin pyrophosphokinase 1 XM_011516034.2 50.7% 50.7% 1_396del;807_808insTTG
34 human 27010 TPK1 thiamin pyrophosphokinase 1 XM_011516043.1 49.6% 32.5% (many diffs)
35 human 27010 TPK1 thiamin pyrophosphokinase 1 NM_001350881.1 47.4% 40.3% (many diffs)
36 human 27010 TPK1 thiamin pyrophosphokinase 1 XM_017011969.1 43.2% 36.3% (many diffs)
37 human 27010 TPK1 thiamin pyrophosphokinase 1 XM_011516035.3 43% 36.6% (many diffs)
38 human 27010 TPK1 thiamin pyrophosphokinase 1 XM_017011972.1 40.8% 37.4% (many diffs)
39 human 27010 TPK1 thiamin pyrophosphokinase 1 XM_011516040.2 40% 37% (many diffs)
40 human 27010 TPK1 thiamin pyrophosphokinase 1 NR_146936.2 14.8% (many diffs)
41 mouse 29807 Tpk1 thiamine pyrophosphokinase XM_006506219.3 53.8% 56.8% (many diffs)
42 mouse 29807 Tpk1 thiamine pyrophosphokinase NM_013861.4 48.5% 51.2% (many diffs)
43 mouse 29807 Tpk1 thiamine pyrophosphokinase XM_006506218.3 32.7% 28.4% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
72
ORF end:
486
ORF length:
414
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61caggcttcac catgctccaa aagaagatag aagaaaaaga cttaaaggtt gatgtgatcg
121tgacactgGG AGGCCTTGCT GGGCGTTTTG ACCAGATTAT GGCATCTGTG AATACCTTGT
181TCCAAGCGAC TCACATCACT CCTTTTCCAA TTATAATAAT CCAAGAGGAA TCGCTGATCT
241ACCTGCTCCA ACCAGGAAAG CACAGGTTGC ATGTAGACAC TGGAATGGAG GGTGATTGGT
301GTGGCCTTAT TCCTGTTGGA CAGCCTTGTA TGCAGGTTAC AACCACAGGC CTCAAGTGGA
361ACCTCACAAA TGATGTGCTT GCTTTTGGAA CATTGGTCAG TACTTCCAAT ACCTACGACG
421GGTCTGGTGT TGTGACTGTG GAAACTGACC ACCCACTCCT CTGGACCATG GCCATCAAAA
481GCTTGTGAGA CCCAGCTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC
541CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA
601TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGATACGCA ACGCCATGAA TTACAAAAAC
661GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt

Download FASTA (ORF) (Full)