Construct: ORF TRCN0000491800
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021455.2_s317c1
- DNA Barcode:
- GCTGTATCTTGTCACCTAGCGTCA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- COMT (1312)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491800
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1312 | COMT | catechol-O-methyltransferase | NM_000754.4 | 100% | 100% | |
| 2 | human | 1312 | COMT | catechol-O-methyltransferase | NM_001135161.2 | 100% | 100% | |
| 3 | human | 1312 | COMT | catechol-O-methyltransferase | NM_001135162.2 | 100% | 100% | |
| 4 | human | 1312 | COMT | catechol-O-methyltransferase | NM_001362828.2 | 100% | 100% | |
| 5 | human | 1312 | COMT | catechol-O-methyltransferase | NM_007310.3 | 81.5% | 81.5% | 0_1ins150 |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 879
- ORF length:
- 813
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaagaat 61 tcaccatgcc ggaggccccg cctctgctgt tggcagctgt gttgctgggc ctggtgctgc 121 tggtggtgct gctgctgctt ctgaggcact ggggctgggg cctgtgcctt atcggctgga 181 acgagttcat cctgcagccc atccacaacc tgctcatggg tgacaccaag gagcagcgca 241 tcctgaacca cgtgctgcag catgcggagc ccgggaacgc acagagcgtg ctggaggcca 301 ttgacaccta ctgcgagcag aaggagtggg ccatgaacgt gggcgacaag aaaggcaaga 361 tcgtggacgc cgtgattcag gagcaccagc cctccgtgct gctggagctg ggggcctact 421 gtggctactc agctgtgcgc atggcccgcc tgctgtcacc aggggcgagg ctcatcacca 481 tcgagatcaa ccccgactgt gccgccatca cccagcggat ggtggatttc gctggcgtga 541 aggacaaggt cacccTTGTG GTTGGAGCGT CCCAGGACAT CATCCCCCAG CTGAAGAAGA 601 AGTATGATGT GGACACACTG GACATGGTCT TCCTCGACCA CTGGAAGGAC CGGTACCTGC 661 CGGACACGCT TCTCTTGGAG GAATGTGGCC TGCTGCGGAA GGGGACAGTG CTACTGGCTG 721 ACAACGTGAT CTGCCCAGGT GCGCCAGACT TCCTAGCACA CGTGCGCGGG AGCAGCTGCT 781 TTGAGTGCAC ACACTACCAA TCGTTCCTGG AATACAGGGA GGTGGTGGAC GGCCTGGAGA 841 AGGCCATCTA CAAGGGCCCA GGCAGCGAAG CAGGGCCCTG AAAGCCAGCT TTCTTGTACA 901 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 961 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 1021 AGGACGAGCT GTATCTTGTC ACCTAGCGTC AACGCGTTAA GTCgacaatc aacctctgga 1081 ttacaaaatt tgtgaaagat t