Construct: ORF TRCN0000491815
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019208.2_s317c1
- DNA Barcode:
- GCGGTGTCGAAAACTGCCTGCACC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TAS2R1 (50834)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491815
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 50834 | TAS2R1 | taste 2 receptor member 1 | NM_019599.2 | 99.8% | 99.6% | 897_898insG |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 75
- ORF end:
- 975
- ORF length:
- 900
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcgc caccatgcta gagtctcacc tcattatcta ttttcttctt gcagtgatac 121 aatttcttct tgggattttc acaaatggca tcattgtggt ggtgaatggc attgacttga 181 tcaagcacag aaaaatggct ccgctggatc tccttctttc ttgtctggca gtttctagaa 241 tttttctgca gttgttcatc ttctacgtta atgtgattgt tatcttcttc atagaattca 301 tcatgtgttc tgcgaattgt gcaattctct tatttataaa tgaattggaa ctttggcttg 361 ccacatggct cggcgttttc tattgtgcca aggttgccag cgtccgtcac ccactcttca 421 tctggttgaa gatgaggata tccaagctgg tcccatggat gatcctgggg tctctgctat 481 atgtatctat gatttgtgtt ttccatagca aatatgcagg gtttatggtc ccatacttcc 541 taaggaaatt tttctcccaa aatgccacaa ttcaaaaaga agatacactG GCTATACAGA 601 TTTTCTCTTT TGTTGCTGAG TTCTCAGTGC CATTGCTTAT CTTCCTTTTT GCTGTTTTGC 661 TCTTGATTTT CTCTCTGGGG AGGCACACCC GGCAAATGAG AAACACAGTG GCCGGCAGCA 721 GGGTTCCTGG CAGGGGTGCA CCCATCAGCG CGTTGCTGTC TATCCTGTCC TTCCTGATCC 781 TCTACTTCTC CCACTGCATG ATAAAAGTTT TTCTCTCTTC TCTAAAGTTT CACATCAGAA 841 GGTTCATCTT TCTGTTCTTC ATCCTTGTGA TTGGTATATA CCCTTCTGGA CACTCTCTCA 901 TCTTAATTTT AGGAAATCCT AAATTGAAAC AAAATGCAAA AAAGTTCCTC CTCCACAGTA 961 AGTGCTGTCA GGACCCAGCT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 1021 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 1081 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGAGCG GTGTCGAAAA CTGCCTGCAC 1141 CACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t