Construct: ORF TRCN0000491830
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017056.2_s317c1
- Derived from:
- ccsbBroadEn_08676
- DNA Barcode:
- CCCTTCGAGGCCAAATATTTTGGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NIT2 (56954)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491830
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 56954 | NIT2 | nitrilase family member 2 | NM_020202.5 | 99.8% | 99.6% | 692T>C |
| 2 | mouse | 52633 | Nit2 | nitrilase family, member 2 | NM_023175.1 | 87.8% | 89.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 894
- ORF length:
- 828
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgac ctctttccgc ttggccctca tccagcttca gatttcttcc atcaaatcag 121 ataacgtcac tcgcgcttgt agcttcatcc gggaggcagc aacgcaagga gccaaaatag 181 tttctttgcc ggaatgcttt aattctccat atggagcgaa atattttcct gaatatgcag 241 agaaaattcc tggtgaatcc acacagaagc tttctgaagt agcaaaggaa tgcagcatat 301 atctcattgg aggctctatc cctgaagagg atgctgggaa attatataac acctgtgctg 361 tgtttgggcc tgatggaact ttactagcaa agtatagaaa gatccatctg tttgacattg 421 atgttcctgg aaaaattaca tttcaagaat ctaaaacatt gagtccgggt gatagtttct 481 ccacatttga tactccttac tgcagagtgg gtctgggcat ctgcTACGAC ATGCGGTTTG 541 CAGAGCTTGC ACAAATCTAC GCACAGAGAG GCTGCCAGCT GTTGGTATAT CCAGGAGCTT 601 TTAATCTGAC CACTGGACCA GCCCATTGGG AGTTACTTCA GCGAAGCCGG GCTGTTGATA 661 ATCAGGTGTA TGTGGCCACA GCCTCTCCTG CCCGGGATGA CAAAGCCTCC TATGTTGCCT 721 GGGGACACAG CACCGTGGTG AACCCTTGGG GGGAGGCTCT AGCCAAAGCT GGCACAGAAG 781 AAGCAATCGT GTATTCAGAC ATAGACCTGA AGAAGCTGGC TGAAATACGC CAGCAAATCC 841 CCGTTTTTAG ACAGAAGCGA TCAGACCTCT ATGCTGTGGA GATGAAAAAG CCCTACCCAA 901 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 961 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 1021 TATCTTGTGG AAAGGACGAC CCTTCGAGGC CAAATATTTT GGGACGCGTT AAGTCgacaa 1081 tcaacctctg gattacaaaa tttgtgaaag att