Construct: ORF TRCN0000491831
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001442.2_s317c1
- Derived from:
- ccsbBroadEn_15796
- DNA Barcode:
- GAGTAGACATACAGTTCGAGTGCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MAT2B (27430)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491831
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 27430 | MAT2B | methionine adenosyltransfer... | NM_182796.2 | 99.8% | 99.6% | 844G>A |
| 2 | human | 27430 | MAT2B | methionine adenosyltransfer... | NM_013283.5 | 95.3% | 93.4% | (many diffs) |
| 3 | mouse | 108645 | Mat2b | methionine adenosyltransfer... | NM_001199274.1 | 89% | 94.1% | (many diffs) |
| 4 | mouse | 108645 | Mat2b | methionine adenosyltransfer... | NM_134017.2 | 84% | 88.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1035
- ORF length:
- 969
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcc tgaaatgcca gaggacatgg agcaggagga agttaacatc cctaatagga 121 gggttctggt tactggtgcc actgggcttc ttggcagagc tgtacacaaa gaatttcagc 181 agaataattg gcatgcagtt ggctgtggtt tcagaagagc aagaccaaaa tttgaacagg 241 ttaatctgtt ggattctaat gcagttcatc acatcattca tgattttcag ccccatgtta 301 tagtacattg tgcagcagag agaagaccag atgttgtaga aaatcagcca gatgctgcct 361 ctcaacttaa tgtggatgct tctgggaatt tagcaaagga agcagctgct gttggagcat 421 ttctcatcta cattagctca gattatgtat ttgatggaac aaatccacct tacagagagg 481 aagacatacc agctccccta aatttgtatg gcaaaacaaa attagatgga gaaaaggctg 541 tcctggagaa caatctagga gctgctgttt tgaggattcc tattctgtat ggggaagttg 601 aaaagctcGA AGAAAGTGCT GTGACTGTTA TGTTTGATAA AGTGCAGTTC AGCAACAAGT 661 CAGCAAACAT GGATCACTGG CAGCAGAGGT TCCCCACACA TGTCAAAGAT GTGGCCACTG 721 TGTGCCGGCA GCTAGCAGAG AAGAGAATGC TGGATCCATC AATTAAGGGA ACCTTTCACT 781 GGTCTGGCAA TGAACAGATG ACTAAGTATG AAATGGCATG TGCAATTGCA GATGCCTTCA 841 ACCTCCCCAG CAGTCACTTA AGACCTATTA CTGACAGCCC TGTCCTAGGA GCACAACGTC 901 CGAGAAATAC TCAGCTTGAC TGCTCCAAAT TGGAGACCTT GGGCATTGGC CAACGAACAC 961 CATTTCGAAT TGGAATCAAA GAATCACTTT GGCCTTTCCT CATTGACAAG AGATGGAGAC 1021 AAACGGTCTT TCATTACCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 1081 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 1141 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA GAGTAGACAT ACAGTTCGAG 1201 TGCCACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt