Construct: ORF TRCN0000491835
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015579.3_s317c1
- Derived from:
- ccsbBroadEn_12971
- DNA Barcode:
- TTGCTCCGTTAACCTAGACAACCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RFT1 (91869)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491835
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 91869 | RFT1 | RFT1 homolog | XR_001740360.2 | 8.5% | 1_3115del;3488_4340del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 441
- ORF length:
- 372
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggatggtccc atagggtcaa gtggagcaca cacggaaggc agtcctcctt 121 ccctcccatg tcctttctct gtccctggca tccacagaaa gggaccttca gtcctcatga 181 gctgcTTCTC TGCAGCTGCT GATCACCCTC CCGGAACCCT GCACCTATCC CGTGCCCACC 241 TGACCCCAGA CTCAAGCATG ATCCTCATTC TACCTGCCTC GGAATCACCT GGATTGCAAG 301 TCAGAACATG GCTTCCCAGG ACCCACTCCA GGCCTTCAGG GTCTGTGTCT CCCAGGAGGA 361 GGGCTCTGAG ATTGGCTTTT CTGGCAAGCC CACGTGGCTT TGATGCCACC AAGGTTGGCA 421 TGCCCCATTG TCTTCCTTCG TTGCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 481 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 541 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGATTGC TCCGTTAACC 601 TAGACAACCA ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt