Construct: ORF TRCN0000491851
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003540.1_s317c1
- Derived from:
- ccsbBroadEn_10465
- DNA Barcode:
- GCGTATTGCCAAGCGGACGCTTGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- OXCT2 (64064)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491851
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 64064 | OXCT2 | 3-oxoacid CoA-transferase 2 | NM_022120.2 | 99.1% | 99.2% | 1008C>A;1540_1551del |
| 2 | human | 192217 | OXCT2P1 | 3-oxoacid CoA-transferase 2... | NR_132962.1 | 83% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1605
- ORF length:
- 1539
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ggcgctgcgg ctcctggcgt cagtgctcgg gcgcggggtc cccgccggcg 121 gctcagggct cgcgctgtcc cagggctgcg cccgctgctt tgccaccagt ccccggctcc 181 gtgccaagtt ctacgcggac ccggtggaga tggtgaagga catctctgac ggggcgaccg 241 tcatgatcgg gggcttcggg ctctgcggga tccccgagaa cctgatcgcc gcgctgctca 301 ggacccgcgt gaaagacctg caggtggtca gcagcaacgt gggcgtggag gacttcggcc 361 tgggcctcct gctggccgcc aggcaggtcc gtcgcatcgt ctgttcctac gtgggcgaga 421 acaccctgtg cgagagccag tacctggcag gagagctgga gctggagctc acgccccagg 481 gcaccctggc cgagcgcatc cgcgcggggg gcgccggggt gcccgccttc tacaccccca 541 cgggctacgg gaccctggtc caggaagggg gcgcccccat ccgctacacc ccggacggcc 601 acctggcgct catgagccag ccccgagagg tgagggagtt caacggcgac cacttccttt 661 tggagcgcgc catccgggca gacttcgccc tggtgaaagg gtggaaggcc gaccgggcag 721 gaaacgtggt cttcaggaga agcgcccgca atttcaacgt gcccatgtgc aaagctgcag 781 acgtcacggc ggtggaggtg gaagagatcg tggaggtggg ggctttcccc ccagaagaca 841 tccacgttcc taacatttat gtagatcgcg tgataaaggg gcagaaatac gagaaacgaa 901 ttgagcgctt aacgatcctg aaagaggaag atggagacgc tggaaaggaa gaggacgcca 961 ggacgcgcat catcagacgc gcagctctgg aatttgagga cggcatgtac gccaatctgg 1021 gcataggcat ccccctgctg gccagcaact tcatcagtcc cagcatgact gtacatcttc 1081 acagtgagaa cgggatcctg ggcctgggcc cgtttcccac ggaagatgag gtggatgccg 1141 acctcatcaa tgcaggcaag cagacggtca cggtgcttcc cgggggctgc ttcttcgcca 1201 gcgacgactc cttcgccatg atccgagggg gacacatcca actaaccatg cttggAGCCA 1261 TGCAGGTTTC CAAATACGGC GACCTGGCGA ACTGGATGAT CCCTGGCAAG AAGGTGAAAG 1321 GCATGGGCGG TGCCATGGAC TTGGTGTCCA GTCAGAAGAC CAGAGTGGTG GTCACCATGC 1381 AGCACTGCAC AAAGGACAAC ACCCCCAAGA TCATGGAGAA ATGCACCATG CCGCTGACCG 1441 GGAAGCGGTG CGTGGACCGC ATCATCACCG AGAAGGCCGT GTTTGACGTG CACAGGAAGA 1501 AAGAGCTGAC GCTGAGGGAG CTCTGGGAGG GCCTGACGGT GGACGACATC AAAAAGAGCA 1561 CGGGGTGTGC CTTTGCTGTG TCCCCGAACC TCAGGCCCAT GCAGTACCCA ACTTTCTTGT 1621 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 1681 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 1741 GAAAGGACGA GCGTATTGCC AAGCGGACGC TTGGACGCGT TAAGTCgaca atcaacctct 1801 ggattacaaa atttgtgaaa gatt