Construct: ORF TRCN0000491851

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF003540.1_s317c1
Derived from:
ccsbBroadEn_10465
DNA Barcode:
GCGTATTGCCAAGCGGACGCTTGG
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
OXCT2 (64064)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000491851
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 64064 OXCT2 3-oxoacid CoA-transferase 2 NM_022120.2 99.1% 99.2% 1008C>A;1540_1551del
2 human 192217 OXCT2P1 3-oxoacid CoA-transferase 2... NR_132962.1 83% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1605
ORF length:
1539
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatggc ggcgctgcgg ctcctggcgt cagtgctcgg gcgcggggtc cccgccggcg
121gctcagggct cgcgctgtcc cagggctgcg cccgctgctt tgccaccagt ccccggctcc
181gtgccaagtt ctacgcggac ccggtggaga tggtgaagga catctctgac ggggcgaccg
241tcatgatcgg gggcttcggg ctctgcggga tccccgagaa cctgatcgcc gcgctgctca
301ggacccgcgt gaaagacctg caggtggtca gcagcaacgt gggcgtggag gacttcggcc
361tgggcctcct gctggccgcc aggcaggtcc gtcgcatcgt ctgttcctac gtgggcgaga
421acaccctgtg cgagagccag tacctggcag gagagctgga gctggagctc acgccccagg
481gcaccctggc cgagcgcatc cgcgcggggg gcgccggggt gcccgccttc tacaccccca
541cgggctacgg gaccctggtc caggaagggg gcgcccccat ccgctacacc ccggacggcc
601acctggcgct catgagccag ccccgagagg tgagggagtt caacggcgac cacttccttt
661tggagcgcgc catccgggca gacttcgccc tggtgaaagg gtggaaggcc gaccgggcag
721gaaacgtggt cttcaggaga agcgcccgca atttcaacgt gcccatgtgc aaagctgcag
781acgtcacggc ggtggaggtg gaagagatcg tggaggtggg ggctttcccc ccagaagaca
841tccacgttcc taacatttat gtagatcgcg tgataaaggg gcagaaatac gagaaacgaa
901ttgagcgctt aacgatcctg aaagaggaag atggagacgc tggaaaggaa gaggacgcca
961ggacgcgcat catcagacgc gcagctctgg aatttgagga cggcatgtac gccaatctgg
1021gcataggcat ccccctgctg gccagcaact tcatcagtcc cagcatgact gtacatcttc
1081acagtgagaa cgggatcctg ggcctgggcc cgtttcccac ggaagatgag gtggatgccg
1141acctcatcaa tgcaggcaag cagacggtca cggtgcttcc cgggggctgc ttcttcgcca
1201gcgacgactc cttcgccatg atccgagggg gacacatcca actaaccatg cttggAGCCA
1261TGCAGGTTTC CAAATACGGC GACCTGGCGA ACTGGATGAT CCCTGGCAAG AAGGTGAAAG
1321GCATGGGCGG TGCCATGGAC TTGGTGTCCA GTCAGAAGAC CAGAGTGGTG GTCACCATGC
1381AGCACTGCAC AAAGGACAAC ACCCCCAAGA TCATGGAGAA ATGCACCATG CCGCTGACCG
1441GGAAGCGGTG CGTGGACCGC ATCATCACCG AGAAGGCCGT GTTTGACGTG CACAGGAAGA
1501AAGAGCTGAC GCTGAGGGAG CTCTGGGAGG GCCTGACGGT GGACGACATC AAAAAGAGCA
1561CGGGGTGTGC CTTTGCTGTG TCCCCGAACC TCAGGCCCAT GCAGTACCCA ACTTTCTTGT
1621ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT
1681AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG
1741GAAAGGACGA GCGTATTGCC AAGCGGACGC TTGGACGCGT TAAGTCgaca atcaacctct
1801ggattacaaa atttgtgaaa gatt

Download FASTA (ORF) (Full)