Construct: ORF TRCN0000491892
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020931.2_s317c1
- DNA Barcode:
- TTCCACCGTAGTGATCTCTAGTCC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- TAAR5 (9038)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491892
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9038 | TAAR5 | trace amine associated rece... | NM_003967.2 | 100% | 100% | |
| 2 | mouse | 215854 | Taar5 | trace amine-associated rece... | NM_001009574.1 | 85.4% | 86.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1083
- ORF length:
- 1011
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgagagct gtcttcatcc aaggtgctga agagcaccct gcggcattct 121 gctaccaggt gaatgggtct tgccccagga cagtacatac tctgggcatc cagttggtca 181 tctacctggc ctgtgcagca ggcatgctga ttatcgtgct agggaatgta tttgtggcat 241 ttgctgtgtc ctacttcaaa gcgcttcaca cgcccaccaa cttcctgctg ctctccctgg 301 ccctggctga catgtttctg ggtctgctgg tgctgcccct cagcaccatt cgctcagtgg 361 agagctgctg gttcttcggg gacttcctct gccgcctgca cacctacctg gacaccctct 421 tctgcctcac ctccatcttc catctctgtt tcatttccat tgaccgccac tgtgccatct 481 gtgaccccct gctctatccc tccaagttca cagtgagggt ggctctcagg tacatcctgg 541 caggatgggg ggtgcccgca gcatacactt cgttattcct ctacacagat gtggtagaga 601 caaggctcag ccagtggctg gaagagatgc cttgtgtggg cagttgccag ctgctgctca 661 ataaattttG GGGCTGGTTA AACTTCCCTT TGTTCTTTGT CCCCTGCCTC ATTATGATCA 721 GCTTGTATGT GAAGATCTTT GTGGTTGCTA CCAGACAGGC TCAGCAGATT ACCACATTGA 781 GCAAAAGCCT GGCTGGGGCT GCCAAGCATG AGAGAAAAGC TGCCAAGACC CTGGGCATTG 841 CTGTGGGCAT ATACCTCTTG TGCTGGCTGC CCTTCACCAT AGACACGATG GTCGACAGCC 901 TCCTTCACTT TATCACACCC CCACTGGTCT TTGACATCTT TATCTGGTTT GCTTACTTCA 961 ACTCAGCCTG CAACCCCATC ATCTATGTCT TTTCCTACCA GTGGTTTCGG AAGGCACTGA 1021 AACTCACACT GAGCCAGAAG GTCTTCTCAC CGCAGACACG CACTGTTGAT TTGTACCAAG 1081 AATAGGACCC AGCTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 1141 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGCCCGTA ACTTGAAAGT ATTTCGATTT 1201 CTTGGCTTTA TATATCTTGT GGAAAGGACG ATTCCACCGT AGTGATCTCT AGTCCACGCG 1261 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt