Construct: ORF TRCN0000491897
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019548.2_s317c1
- DNA Barcode:
- AACTGCACCTTATCCGGTTTGACC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ACKR2 (1238)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491897
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1238 | ACKR2 | atypical chemokine receptor 2 | NM_001296.5 | 99.8% | 99.4% | 1118A>C;1152_1153insG |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 75
- ORF end:
- 1230
- ORF length:
- 1155
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcgc caccatggcc gccactgcct ctccgcagcc actcgccact gaggatgccg 121 attctgagaa tagcagcttc tattactatg actacctgga tgaagtggcc ttcatgctct 181 gcaggaagga tgcagtggtg tcctttggca aagtcttcct cccagtcttc tatagcctga 241 tttttgtgtt gggcctcagc gggaacctcc ttcttctcat ggtcttgctc cgttacgtgc 301 ctcgcaggcg gatggttgag atctatctgc tgaatctggc catctccaac cttctgtttc 361 tggtgacact gcccttctgg ggcatctccg tggcctggca ttgggtcttc gggagtttct 421 tgtgcaagat ggtgagcact ctttatacta ttaactttta cagtggcatc tttttcatta 481 gctgcatgag cctggacaag tacctggaga tcgttcatgc tcagccctac cacaggctga 541 ggacccgggc caagagcctg ctccttgcta ccatagtatg ggctgtgtcc ctggccgtct 601 ccatccctga tatggtcttt gtacagacac atgaaaatcc caagggtgtg tggaactgcc 661 acgcagattt cggcgggcat gggaccattt ggaagctctt cctccgcttc cagcagaacc 721 tcctagggtt tctccttcca ctccttgcca tgatcttctt ctactcccgt attggttgtg 781 tcttggtgag gctgaggccc gcaggccagg gccgggcttt aaaaatagct gcagccttgg 841 tggtggcctt cttcgtgcta tggttcccat acaatctcac cttgtttctg catacgctgt 901 tggaccTGCA AGTATTCGGG AACTGTGAGG TCAGCCAGCA TCTAGACTAC GCACTCCAGG 961 TAACAGAGAG CATCGCCTTC CTTCACTGCT GCTTTTCCCC CATCCTGTAT GCCTTCTCCA 1021 GTCACCGCTT CCGCCAGTAC CTGAAGGCTT TCCTGGCTGC CGTGCTTGGA TGGCACCTGG 1081 CACCTGGCAC TGCCCAGGCC TCATTATCCA GCTGTTCTGA GAGCAGCATA CTTACTGCCC 1141 AAGAGGAAAT GACTGGCATG AATGACCTTG GAGAGAGGCA GTCTGAGAAC TCCCCTAACA 1201 AGGAGGATGT GGGGAATAAA TCAGCCGACC CAGCTTTCTT GTACAAAGTG GTTGATATCG 1261 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 1321 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GAAACTGCAC 1381 CTTATCCGGT TTGACCACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 1441 aagatt