Construct: ORF TRCN0000491910
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015699.1_s317c1
- Derived from:
- ccsbBroadEn_01725
- DNA Barcode:
- TTTCCACCTAACCCTATCCAGCCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TUBG1 (7283)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491910
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7283 | TUBG1 | tubulin gamma 1 | NM_001070.5 | 100% | 100% | |
| 2 | human | 27175 | TUBG2 | tubulin gamma 2 | NM_016437.3 | 94.6% | 97.7% | (many diffs) |
| 3 | human | 27175 | TUBG2 | tubulin gamma 2 | NM_001320509.2 | 92.8% | 95.8% | (many diffs) |
| 4 | human | 7283 | TUBG1 | tubulin gamma 1 | XM_024450904.1 | 83.8% | 83.8% | 844_1104del |
| 5 | human | 27175 | TUBG2 | tubulin gamma 2 | XM_024450695.1 | 72.7% | 45.1% | (many diffs) |
| 6 | human | 27175 | TUBG2 | tubulin gamma 2 | XM_024450694.1 | 71.6% | 44.5% | (many diffs) |
| 7 | human | 27175 | TUBG2 | tubulin gamma 2 | XM_024450699.1 | 71.2% | 63.6% | (many diffs) |
| 8 | human | 27175 | TUBG2 | tubulin gamma 2 | XM_024450696.1 | 67.9% | 53.3% | (many diffs) |
| 9 | human | 27175 | TUBG2 | tubulin gamma 2 | XM_011524614.3 | 62.6% | 62.7% | (many diffs) |
| 10 | human | 27175 | TUBG2 | tubulin gamma 2 | XM_017024453.2 | 62.6% | 62.7% | (many diffs) |
| 11 | human | 27175 | TUBG2 | tubulin gamma 2 | XM_017024454.2 | 58% | 58.7% | (many diffs) |
| 12 | human | 27175 | TUBG2 | tubulin gamma 2 | XM_017024455.2 | 58% | 58.7% | (many diffs) |
| 13 | human | 27175 | TUBG2 | tubulin gamma 2 | XM_017024456.2 | 58% | 58.7% | (many diffs) |
| 14 | human | 27175 | TUBG2 | tubulin gamma 2 | XM_024450703.1 | 58% | 58.7% | (many diffs) |
| 15 | human | 27175 | TUBG2 | tubulin gamma 2 | XM_024450697.1 | 48.1% | 21.6% | (many diffs) |
| 16 | human | 27175 | TUBG2 | tubulin gamma 2 | XM_024450698.1 | 48.1% | 21.6% | (many diffs) |
| 17 | human | 27175 | TUBG2 | tubulin gamma 2 | XM_024450700.1 | 38.2% | 22.8% | (many diffs) |
| 18 | human | 27175 | TUBG2 | tubulin gamma 2 | XM_024450701.1 | 38.2% | 22.8% | (many diffs) |
| 19 | human | 27175 | TUBG2 | tubulin gamma 2 | XM_024450702.1 | 38.2% | 22.8% | (many diffs) |
| 20 | mouse | 103733 | Tubg1 | tubulin, gamma 1 | NM_134024.2 | 92.7% | 98.8% | (many diffs) |
| 21 | mouse | 103768 | Tubg2 | tubulin, gamma 2 | NM_134028.2 | 91.5% | 96.8% | (many diffs) |
| 22 | mouse | 103733 | Tubg1 | tubulin, gamma 1 | XM_017314211.1 | 70.2% | 76.1% | (many diffs) |
| 23 | mouse | 103768 | Tubg2 | tubulin, gamma 2 | XM_006531917.2 | 55.7% | 58.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1419
- ORF length:
- 1353
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcc gagggaaatc atcaccctac agttgggcca gtgcggcaat cagattgggt 121 tcgagttctg gaaacagctg tgcgccgagc atggtatcag ccccgagggc atcgtggagg 181 agttcgccac cgagggcact gaccgcaagg acgtcttttt ctaccaggca gacgatgagc 241 actacatccc ccgggccgtg ctgctggact tggaaccccg ggtgatccac tccatcctca 301 actcccccta tgccaagctc tacaacccag agaacatcta cctgtcggaa catggaggag 361 gagctggcaa caactgggcc agcggattct cccagggaga aaagatccat gaggacattt 421 ttgacatcat agaccgggag gcagatggta gtgacagtct agagggcttt gtgctgtgtc 481 actccattgc tggggggaca ggctctggac tgggttccta cctcttagaa cggctgaatg 541 acaggtatcc taagaagctg gtgcagacat actcagtgtt tcccaaccag gacgagatga 601 gcgatgtggt ggtccagcct tacaattcac tcctcacact caagaggctg acgcagaatg 661 cagactgtgt ggtggtgctg gacaacacag ccctgaaccg gattgccaca gaccgcctgc 721 acatccagaa cccatccttc tcccagatca accagctggt gtctaccatc atgtcagcca 781 gcaccaccac cctgcgctac cctggctaca tgaacaatga cctcatcggc ctcatcgcct 841 cgctcattcc caccccacgg ctccacttcc tcatgaccgg ctacacccct ctcactacgg 901 accagtcagt ggccagcgtg aggaagacca cggtcctgga tgtcatgagg cggctgctgc 961 agcccaagaa cgtgatggtg tccacaggcc gagaccgcca gaccaaccac tgcTACATCG 1021 CCATCCTCAA CATCATCCAG GGAGAGGTGG ACCCCACCCA GGTCCACAAG AGCTTGCAGA 1081 GGATCCGGGA ACGCAAGTTG GCCAACTTCA TCCCGTGGGG CCCCGCCAGC ATCCAGGTGG 1141 CCCTGTCGAG GAAGTCTCCC TACCTGCCCT CGGCCCACCG GGTCAGCGGG CTCATGATGG 1201 CCAACCACAC CAGCATCTCC TCGCTCTTCG AGAGAACCTG TCGCCAGTAT GACAAGCTGC 1261 GTAAGCGGGA GGCCTTCCTG GAGCAGTTCC GCAAGGAGGA CATGTTCAAG GACAACTTTG 1321 ATGAGATGGA CACATCCAGG GAGATTGTGC AGCAGCTCAT CGATGAGTAC CATGCGGCCA 1381 CACGGCCAGA CTACATCTCC TGGGGCACCC AGGAGCAGTG CCCAACTTTC TTGTACAAAG 1441 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 1501 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 1561 ACGATTTCCA CCTAACCCTA TCCAGCCGAC GCGTTAAGTC gacaatcaac ctctggatta 1621 caaaatttgt gaaagatt