Construct: ORF TRCN0000491932
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003637.1_s317c1
- Derived from:
- ccsbBroadEn_15946
- DNA Barcode:
- GTGCTAAGAAAGCCAAGCTGCATA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- WDR18 (57418)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491932
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 57418 | WDR18 | WD repeat domain 18 | NM_001372085.1 | 99.7% | 99.7% | 180T>C;201C>G;514G>A |
| 2 | human | 57418 | WDR18 | WD repeat domain 18 | NM_024100.4 | 99.7% | 99.7% | 180T>C;201C>G;514G>A |
| 3 | human | 57418 | WDR18 | WD repeat domain 18 | NM_001372086.1 | 82% | 81.9% | 0_1ins231;283G>A |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1362
- ORF length:
- 1296
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ggcgcccatg gaggtggccg tgtgtacgga ctcggcggcc ccgatgtgga 121 gctgcatcgt gtgggaactt cactcgggcg ccaacctgct cacctaccgc ggcggccagg 181 cgggaccccg cggcctggcg ctgctcaatg gcgagtatct gctggcggcg cagctgggca 241 agaactacat cagcgcctgg gagctgcagc ggaaggacca gctccagcag aagatcatgt 301 gccccgggcc tgtcacctgt ctgactgcat cacccaatgg tctctacgtc ctggcaggag 361 ttgcagaaag catccacctg tgggaggtct ccaccgggaa ccttctggtc atcctgagtc 421 gacactacca ggacgtctcc tgccttcagt tcacagggga cagcagccac ttcatctcag 481 ggggcaagga ctgcctggtg ctggtttgga gcctctgcag cgtgctgcag gccgacccct 541 ccaggattcc ggcgcccagg cacgtctggt ctcaccacac gctccccatc acggacctgc 601 actgcggctt tgggggcccc ctggcccggg tggccacctc ctcactggac cagacggtga 661 agctatggga ggtctcctcg ggggagctgc tgctctccgt cctctttgac gtgtccatca 721 tggcagtgac catggacctg gctgagcacc atatgttctg cgggggcagt gagggctcca 781 tcttccaggt cgacctcttc acctggcccg gacagaggga gaggagcttc cacccagagc 841 aggacgccgg gaaggtcttc aaagggcaca ggaaccaggt gacttgcctg tcagtgtcca 901 ctgacggcag cgtgctgctc tcaggctccc acgacgagac cgtgcgccTC TGGGACGTGC 961 AGAGCAAGCA GTGCATCCGG ACGGTGGCCC TCAAAGGCCC AGTCACCAAT GCCGCCATCC 1021 TGCTGGCGCC CGTCAGCATG CTGAGCTCAG ACTTCAGGCC CAGCCTGCCG CTGCCCCACT 1081 TCAACAAGCA CCTGCTGGGC GCCGAGCACG GGGACGAGCC GCGCCACGGG GGCCTCACTC 1141 TGCGCCTGGG CCTCCACCAG CAGGGCTCGG AGCCCAGCTA CCTGGACCGC ACGGAGCAGC 1201 TGCAGGCCGT CCTGTGCAGC ACCATGGAGA AGAGCGTGCT CGGCGGCCAG GACCAGCTGC 1261 GCGTCCGTGT GACGGAGCTG GAGGACGAGG TGCGCAACCT GCGCAAGATC AATCGGGACC 1321 TGTTCGACTT CTCCACGCGC TTCATCACGC GGCCGGCCAA GTGCCCAACT TTCTTGTACA 1381 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 1441 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 1501 AGGACGAGTG CTAAGAAAGC CAAGCTGCAT AACGCGTTAA GTCgacaatc aacctctgga 1561 ttacaaaatt tgtgaaagat t