Construct: ORF TRCN0000491939
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020736.2_s317c1
- DNA Barcode:
- TAAATTGCGTAAATAACATGGGTG
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- KIT (3815)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491939
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3815 | KIT | KIT proto-oncogene, recepto... | NM_000222.2:c.1727T>C | 99.9% | 100% | 2290C>T |
| 2 | human | 3815 | KIT | KIT proto-oncogene, recepto... | NM_000222.2 | 99.9% | 99.8% | 1727T>C;2290C>T |
| 3 | human | 3815 | KIT | KIT proto-oncogene, recepto... | XM_005265740.1 | 99.8% | 99.7% | 754_756delCAG;1730T>C;2293C>T |
| 4 | human | 3815 | KIT | KIT proto-oncogene, recepto... | XM_017008178.1 | 99.8% | 99.7% | 1727T>C;2140_2141insGCA;2287C>T |
| 5 | human | 3815 | KIT | KIT proto-oncogene, recepto... | XM_005265741.1 | 99.7% | 99.6% | (many diffs) |
| 6 | human | 3815 | KIT | KIT proto-oncogene, recepto... | NM_001093772.1 | 99.5% | 99.4% | 1524_1525insAAAGGTAACAAC;1715T>C;2278C>T |
| 7 | human | 3815 | KIT | KIT proto-oncogene, recepto... | XM_005265742.3 | 99.4% | 99.3% | (many diffs) |
| 8 | human | 3815 | KIT | KIT proto-oncogene, recepto... | NM_000222.2:c.1673_1687del | 99.4% | 99.3% | 1670_1671insGAAGGTTGTTGAGGA;1712T>C;2275C>T |
| 9 | human | 3815 | KIT | KIT proto-oncogene, recepto... | XM_017008180.1 | 99.4% | 99.3% | (many diffs) |
| 10 | human | 3815 | KIT | KIT proto-oncogene, recepto... | XM_017008179.1 | 99.3% | 99.2% | (many diffs) |
| 11 | human | 3815 | KIT | KIT proto-oncogene, recepto... | NM_000222.2:c.1648_1674del | 99% | 98.9% | 1645_1646ins27;1700T>C;2263C>T |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 3000
- ORF length:
- 2928
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgagaggc gctcgcggcg cctgggattt tctctgcgtt ctgctcctac 121 tgcttcgcgt ccagacaggc tcttctcaac catctgtgag tccaggggaa ccgtctccac 181 catccatcca tccaggaaaa tcagacttaa tagtccgcgt gggcgacgag attaggctgt 241 tatgcactga tccgggcttt gtcaaatgga cttttgagat cctggatgaa acgaatgaga 301 ataagcagaa tgaatggatc acggaaaagg cagaagccac caacaccggc aaatacacgt 361 gcaccaacaa acacggctta agcaattcca tttatgtgtt tgttagagat cctgccaagc 421 ttttccttgt tgaccgctcc ttgtatggga aagaagacaa cgacacgctg gtccgctgtc 481 ctctcacaga cccagaagtg accaattatt ccctcaaggg gtgccagggg aagcctcttc 541 ccaaggactt gaggtttatt cctgacccca aggcgggcat catgatcaaa agtgtgaaac 601 gcgcctacca tcggctctgt ctgcattgtt ctgtggacca ggagggcaag tcagtgctgt 661 cggaaaaatt catcctgaaa gtgaggccag ccttcaaagc tgtgcctgtt gtgtctgtgt 721 ccaaagcaag ctatcttctt agggaagggg aagaattcac agtgacgtgc acaataaaag 781 atgtgtctag ttctgtgtac tcaacgtgga aaagagaaaa cagtcagact aaactacagg 841 agaaatataa tagctggcat cacggtgact tcaattatga acgtcaggca acgttgacta 901 tcagttcagc gagagttaat gattctggag tgttcatgtg ttatgccaat aatacttttg 961 gatcagcaaa tgtcacaaca accttggaag tagtagataa aggattcatt aatatcttcc 1021 ccatgataaa cactacagta tttgtaaacg atggagaaaa tgtagatttg attgttgaat 1081 atgaagcatt ccccaaacct gaacaccagc agtggatcta tatgaacaga accttcactg 1141 ataaatggga agattatccc aagtctgaga atgaaagtaa tatcagatac gtaagtgaac 1201 ttcatctaac gagattaaaa ggcaccgaag gaggcactta cacattccta gtgtccaatt 1261 ctgacgtcaa tgctgccata gcatttaatg tttatgtgaa tacaaaacca gaaatcctga 1321 cttacgacag gctcgtgaat ggcatgctcc aatgtgtggc agcaggattc ccagagccca 1381 caatagattg gtatttttgt ccaggaactg agcagagatg ctctgcttct gtactgccag 1441 tggatgtgca gacactaaac tcatctgggc caccgtttgg aaagctagtg gttcagagtt 1501 ctatagattc tagtgcattc aagcacaatg gcacggttga atgtaaggct tacaacgatg 1561 tgggcaagac ttctgcctat tttaactttg catttaaagg taacaacaaa gagcaaatcc 1621 atccccacac cctgttcact cctttgctga ttggtttcgt aatcgtagct ggcatgatgt 1681 gcattattgt gatgattctg acctacaaat atttacagaa acccatgtat gaagtacagt 1741 ggaaggttgt tgaggagata aatggaaaca attatgttta catagaccca acacaacctc 1801 cttatgatca caaatgggag tttcccagaa acaggctgag ttttgggaaa accctgggtg 1861 ctggagcttt cgggaaggtt gttgaggcaa ctgcttatgg cttaattaag tcagatgcgg 1921 ccatgactgt cgctgtaaag atgctcaagc cgagtgccca tttgacagaa cgggaagccc 1981 tcatgtctga actcaaagtc ctgagttacc ttggtaatca catgaatatt gtgaatctac 2041 ttggagcctg caccattgga gggcccaccc tggtcattac agaatattgt tgctatggtg 2101 atcttttgaa ttttttgaga agaaaacgtg attcatttat ttgttcaaag caggaagatc 2161 atgcagaagc tgcactttat aagaatcttc tgcattcaaa ggagtcttcc tgcagcgata 2221 gtactaatga gtacatggac atgaaacctg gagtttctta tgttgtccca accaaggccg 2281 acaaaaggag atctgtgaga ataggctcat acatagaaag agatgtgact cccgccatca 2341 tggaggatga cgagttggcc ttagacttag aagacttgct gagcttttct taccaggtgg 2401 caaagggcat ggctttcctc gcctccaaga attgtattca cagagacttg gcagccagaa 2461 atatcctcct tactcatggt cggatcacaa agatttgtga ttttggtcta gccagagaca 2521 tcaagaatga ttctaattat gtggttaaag gaaacgctcg actacctgtg aagtggatgg 2581 cacctgaaag catttTCAAC TGTGTATACA CGTTTGAAAG TGACGTCTGG TCCTATGGGA 2641 TTTTTCTTTG GGAGCTGTTC TCTTTAGGAA GCAGCCCCTA TCCTGGAATG CCGGTCGATT 2701 CTAAGTTCTA CAAGATGATC AAGGAAGGCT TCCGGATGCT CAGCCCTGAA CACGCACCTG 2761 CTGAAATGTA TGACATAATG AAGACTTGCT GGGATGCAGA TCCCCTAAAA AGACCAACAT 2821 TCAAGCAAAT TGTTCAGCTA ATTGAGAAGC AGATTTCAGA GAGCACCAAT CATATTTACT 2881 CCAACTTAGC AAACTGCAGC CCCAACCGAC AGAAGCCCGT GGTAGACCAT TCTGTGCGGA 2941 TCAATTCTGT CGGCAGCACC GCTTCCTCCT CCCAGCCTCT GCTTGTGCAC GACGATGTCT 3001 AGAACCCAGC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 3061 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 3121 GGCTTTATAT ATCTTGTGGA AAGGACGATA AATTGCGTAA ATAACATGGG TGACGCGTTA 3181 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt