Construct: ORF TRCN0000491985
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019895.2_s317c1
- DNA Barcode:
- CTTTAACATCAATATTCACTGGAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DDX21 (9188)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491985
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9188 | DDX21 | DExD-box helicase 21 | NM_004728.4 | 99.9% | 99.8% | 2349_2350insG |
| 2 | human | 9188 | DDX21 | DExD-box helicase 21 | XM_011540336.1 | 93% | 92.9% | 1384_1385ins162;2187_2188insG |
| 3 | human | 9188 | DDX21 | DExD-box helicase 21 | NM_001256910.1 | 91.2% | 91.1% | 0_1ins204;2145_2146insG |
| 4 | human | 9188 | DDX21 | DExD-box helicase 21 | XM_017016910.2 | 84.3% | 84.3% | 0_1ins204;1180_1181ins162;1983_1984insG |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2421
- ORF length:
- 2352
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcaccat gccgggaaaa ctccgtagtg acgctggttt ggaatcagac accgcaatga 121 aaaaagggga gacactgcga aagcaaaccg aggagaaaga gaaaaaagag aagccaaaat 181 ctgataagac tgaagagata gcagaagagg aagaaactgt tttccccaaa gctaaacaag 241 ttaaaaagaa agcagagcct tctgaagttg acatgaattc tcctaaatcc aaaaaggcaa 301 aaaagaaaga ggagccatct caaaatgaca tttctcctaa aaccaaaagt ttgagaaaga 361 aaaaggagcc cattgaaaag aaagtggttt cttctaaaac caaaaaagtg acaaaaaatg 421 aggagccttc tgaggaagaa atagatgctc ctaagcccaa gaagatgaag aaagaaaagg 481 aaatgaatgg agaaactaga gagaaaagcc ccaaactgaa gaatggattt cctcatcctg 541 aaccggactg taaccccagt gaagctgcca gtgaagaaag taacagtgag atagagcagg 601 aaatacctgt ggaacaaaaa gaaggcgctt tctctaattt tcccatatct gaagaaacta 661 ttaaacttct caaaggccga ggagtgacct tcctatttcc tatacaagca aagacattcc 721 atcatgttta cagcgggaag gacttaattg cacaggcacg gacaggaact gggaagacat 781 tctcctttgc catccctttg attgagaaac ttcatgggga actgcaagac aggaagagag 841 gccgtgcccc tcaggtactg gttcttgcac ctacaagaga gttggcaaat caagtaagca 901 aagacttcag tgacatcaca aaaaagctgt cagtggcttg tttttatggt ggaactccct 961 atggaggtca atttgaacgc atgaggaatg ggattgatat cctggttgga acaccaggtc 1021 gtatcaaaga ccacatacag aatggcaaac tagatctcac caaacttaag catgttgtcc 1081 tggatgaagt ggaccagatg ttggatatgg gatttgctga tcaagtggaa gagattttaa 1141 gtgtggcata caagaaagat tctgaagaca atccccaaac attgcttttt tctgcaactt 1201 gccctcattg ggtatttaat gttgccaaga aatacatgaa atctacatat gaacaggtgg 1261 acctgattgg taaaaagact cagaaaacgg caataactgt ggagcatctg gctattaagt 1321 gccactggac tcagagggca gcagttattg gggatgtcat ccgagtatat agtggtcatc 1381 aaggacgcac tatcatcttt tgtgaaacca agaaagaagc ccaggagctg tcccagaatt 1441 cagctataaa gcaggatgct cagtccttgc atggagacat tccacagaag caaagggaaa 1501 tcaccctgaa aggttttaga aatggtagtt ttggagtttt ggtggcaacc aatgttgctg 1561 cacgtgggtt agacatccct gaggttgatt tggttataca aagctctcca ccaaaggatg 1621 tagagtccta cattcatcga tccgggcgga caggcagagc tggaaggacg ggggtgtgca 1681 tctgctttta tcagcacaag gaagaatatc agttagtaca agtggagcaa aaagcgggaa 1741 ttaagttcaa acgaataggt gttccttctg caacagaaat aataaaagct tccagcaaag 1801 atgccatcag gcttttggat tccgtgcctc ccactgccat tagtcacttc aaacaatcag 1861 ctgagaagct gatagaggag aagggagctg tggaagctct ggcagcagca ctggcccata 1921 tttcaggtgc cacgtccgta gaccagcgct ccttgatcaa ctcaaatgtg ggttttgtga 1981 ccatgatctt gcagtgctca attgaaatgc caaatattag ttatgcttgg aaagaactta 2041 aagagcagct gggcgaggag attgattcca aagtgaaggg aatggttttt ctcaaaggaa 2101 agctGGGTGT TTGCTTTGAT GTACCTACCG CATCAGTAAC AGAAATACAG GAGAAATGGC 2161 ATGATTCACG ACGCTGGCAG CTCTCTGTGG CCACAGAGCA ACCAGAACTG GAAGGACCAC 2221 GGGAAGGATA TGGAGGCTTC AGGGGACAGC GGGAAGGCAG TCGAGGCTTC AGGGGACAGC 2281 GGGACGGAAA CAGAAGATTC AGAGGACAGC GGGAAGGCAG TAGAGGCCCG AGAGGACAGC 2341 GATCAGGAGG TGGCAACAAA AGTAACAGAT CCCAAAACAA AGGCCAGAAG CGGAGTTTCA 2401 GTAAAGCATT TGGTCAAGAC CCAGCTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 2461 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 2521 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGACTTTAAC ATCAATATTC 2581 ACTGGAAACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt