Construct: ORF TRCN0000491993
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014544.1_s317c1
- Derived from:
- ccsbBroadEn_13472
- DNA Barcode:
- CGCAGACGAAATAGTCTAACCCAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- H2BC1 (255626)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491993
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 255626 | H2BC1 | H2B clustered histone 1 | NM_170610.2 | 99.2% | 100% | 129C>T;198C>T;381G>A |
| 2 | human | 8348 | H2BC17 | H2B clustered histone 17 | NM_003527.4 | 77.3% | 85% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 450
- ORF length:
- 381
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gccggaggtg tcatctaaag gtgctaccaT TTCCAAGAAG GGCTTTAAGA 121 AAGCTGTCGT TAAGACCCAG AAAAAGGAAG GCAAAAAGCG CAAGAGGACC CGTAAGGAGA 181 GTTATTCTAT TTACATTTAC AAAGTGCTAA AGCAGGTCCA TCCGGACACT GGCATCTCTT 241 CGAAAGCTAT GAGCATTATG AATTCTTTCG TCACTGATAT CTTTGAGCGT ATAGCGAGCG 301 AGGCATCACG TTTGGCTCAC TACAGCAAGC GCTCCACCAT TTCTTCCAGA GAGATTCAGA 361 CAGCAGTGCG CTTGCTACTG CCGGGAGAGC TGGCTAAACA TGCTGTGTCT GAGGGCACCA 421 AGGCTGTCAC TAAGTACACC AGCTCCAAAT TGCCAACTTT CTTGTACAAA GTGGTTGATA 481 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 541 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGACGCAG 601 ACGAAATAGT CTAACCCACA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 661 tgaaagatt