Construct: ORF TRCN0000492009
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021356.2_s317c1
- DNA Barcode:
- AGTTCGAAATACGCATGCGATAAA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- CTRC (11330)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000492009
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 11330 | CTRC | chymotrypsin C | NM_007272.3 | 100% | 100% | |
| 2 | human | 11330 | CTRC | chymotrypsin C | XM_011540550.1 | 66.7% | 56.3% | 493_494ins146;659_840del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 876
- ORF length:
- 804
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgttgggc atcactgtcc tcgctgcgct cttggcctgt gcctccagct 121 gtggggtgcc cagcttcccg cccaacctat ccgcccgagt ggtgggagga gaggatgccc 181 ggccccacag ctggccctgg cagatctccc tccagtacct caagaacgac acgtggaggc 241 atacgtgtgg cgggactttg attgctagca acttcgtcct cactgccgcc cactgcatca 301 gcaacacccg gacctaccgt gtggccgtgg gaaagaacaa cctggaggtg gaagacgaag 361 aaggatccct gtttgtgggt gtggacacca tccacgtcca caagagatgg aatgccctcc 421 tgttgcgcaa tgatattgcc ctcatcaagc ttgcagagca tgtggagctg agtgacacca 481 tccaggtggc ctgcctgcca gagaaggact ccctgcTCCC CAAGGACTAC CCCTGCTATG 541 TCACCGGCTG GGGCCGCCTC TGGACCAACG GCCCCATTGC TGATAAGCTG CAGCAGGGCC 601 TGCAGCCCGT GGTGGATCAC GCCACGTGCT CCAGGATTGA CTGGTGGGGC TTCAGGGTGA 661 AGAAAACCAT GGTGTGCGCT GGGGGCGATG GCGTCATCTC AGCCTGCAAT GGGGACTCCG 721 GTGGCCCACT GAACTGCCAG TTGGAGAACG GTTCCTGGGA GGTGTTTGGC ATCGTCAGCT 781 TTGGCTCCCG GCGGGGCTGC AACACCCGCA AGAAGCCGGT AGTCTACACC CGGGTGTCCG 841 CCTACATCGA CTGGATCAAC GAGAAAATGC AGCTGTAGAA CCCAGCTTTC TTGTACAAAG 901 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 961 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 1021 ACGAAGTTCG AAATACGCAT GCGATAAAAC GCGTTAAGTC gacaatcaac ctctggatta 1081 caaaatttgt gaaagatt