Construct: ORF TRCN0000492038
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012698.1_s317c1
- Derived from:
- ccsbBroadEn_03988
- DNA Barcode:
- ACCCTCCAAAGATGTTCAACTGCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MRPS26 (64949)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000492038
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 64949 | MRPS26 | mitochondrial ribosomal pro... | NM_030811.4 | 100% | 100% |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 681
- ORF length:
- 615
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgct acgcgcgctg agccgcctgg gcgcggggac cccgtgcagg ccccgggccc 121 ctctggtgct gccagcgcgc ggccgcaaga cccgccacga cccgctggcc aaatccaaga 181 tcgagcgagt gaacatgccg cccgcggtgg accctgcgga gttcttcgtg ctgatggagc 241 gttaccagca ctaccgccag accgtgcgcg cccTCAGGAT GGAGTTCGTG TCCGAGGTGC 301 AGAGGAAGGT GCACGAGGCC CGAGCCGGGG TTCTGGCGGA GCGCAAGGCC CTGAAGGACG 361 CCGCCGAGCA CCGCGAGCTG ATGGCCTGGA ACCAGGCGGA GAACCGGCGG CTGCACGAGC 421 TGCGGATAGC GAGGCTGCGG CAGGAGGAGC GGGAGCAGGA GCAGCGGCAG GCGTTGGAGC 481 AGGCCCGCAA GGCCGAAGAG GTGCAGGCCT GGGCGCAGCG CAAGGAGCGG GAAGTGCTGC 541 AGCTGCAGGA AGAGGTGAAA AACTTCATCA CCCGAGAGAA CCTGGAGGCA CGGGTGGAAG 601 CAGCATTGGA CTCCCGGAAG AACTACAACT GGGCCATCAC CAGAGAGGGG CTGGTGGTCA 661 GGCCACAACG CAGGGACTCC TACCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 721 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 781 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGAACCC TCCAAAGATG 841 TTCAACTGCA ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt