Construct: ORF TRCN0000492040
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019739.2_s317c1
- DNA Barcode:
- CACTGCACGATGGGTGCTCACTTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SCD5 (79966)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000492040
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 79966 | SCD5 | stearoyl-CoA desaturase 5 | NM_001037582.3 | 100% | 100% | |
| 2 | human | 79966 | SCD5 | stearoyl-CoA desaturase 5 | NM_024906.2 | 69.3% | 59% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 99
- ORF end:
- 1089
- ORF length:
- 990
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggctccgc ggccgccccc ttcacctcta gaccagccat gccaggcccg gccaccgacg 121 cggggaagat ccctttctgc gacgccaagg aagaaatccg tgccgggctc gaaagctctg 181 agggcggcgg cggcccggag aggccaggcg cgcgcgggca gcggcagaac atcgtctgga 241 ggaatgtcgt cctgatgagc ttgctccact tgggggccgt gtactccctg gtgctcatcc 301 ccaaagccaa gccactcact ctgctctggg cctacttctg cttcctcctg gccgctctgg 361 gtgtgacagc tggtgcccat cgcttgtgga gccacaggtc ctaccgggcc aagctgcctc 421 tgaggatatt tctggctgtc gccaactcca tggctttcca gaatgacatc ttcgagtggt 481 ccagggacca ccgagcccac cacaagtact cagagacgga tgctgacccc cacaatgccc 541 gccggggctt cttcttctcc catattgggt ggctgtttgt tcgcaagcat cgagatgtta 601 ttgagaaggg gagaaagctt gacgtcactg acctgcttgc tgatcctgtg gtccggatcc 661 agagaaagta ctataagatc tccgtggtgc tcatgtgctt tgtggtcccc acgctggtgc 721 cctggtacat ctGGGGAGAG AGTCTGTGGA ATTCCTACTT CTTGGCCTCT ATTCTCCGCT 781 ATACCATCTC ACTCAACATC AGCTGGCTGG TCAACAGCGC CGCCCACATG TATGGAAACC 841 GGCCCTATGA CAAGCACATC AGCCCTCGGC AGAACCCACT CGTCGCTCTG GGTGCCATTG 901 GTGAAGGCTT CCATAATTAC CATCACACCT TTCCCTTTGA CTACTCTGCG AGTGAATTTG 961 GCTTAAATTT TAACCCAACC ACCTGGTTCA TTGATTTCAT GTGCTGGCTG GGGCTGGCCA 1021 CTGACCGCAA ACGGGCAACC AAGCCGATGA TCGAGGCCCG GAAGGCCAGG ACTGGAGACA 1081 GCAGTGCTAA GGGTGGGCGC GCCGACCCAG CTTTCTTGTA CAAAGTGGTT GATATCGGTA 1141 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 1201 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAC ACTGCACGAT 1261 GGGTGCTCAC TTCACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 1321 att