Construct: ORF TRCN0000492140
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019754.2_s317c1
- DNA Barcode:
- ATACACCAGGATCTAATTAAAAGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GPR55 (9290)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000492140
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9290 | GPR55 | G protein-coupled receptor 55 | NM_005683.4 | 99.8% | 99.6% | 957_958insG |
| 2 | human | 9290 | GPR55 | G protein-coupled receptor 55 | XM_005246952.4 | 99.8% | 99.6% | 957_958insG |
| 3 | human | 9290 | GPR55 | G protein-coupled receptor 55 | XM_011512175.3 | 99.8% | 99.6% | 957_958insG |
| 4 | human | 9290 | GPR55 | G protein-coupled receptor 55 | XM_011512176.2 | 99.8% | 99.6% | 957_958insG |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1029
- ORF length:
- 960
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcaccat gagtcagcaa aacaccagtg gggactgcct gtttgacggt gtcaacgagc 121 tgatgaaaac cctacagttt gcagtccaca tccccacctt cgtcctgggc ctgctcctca 181 acctgctggc catccatggc ttcagcacct tccttaagaa caggtggccc gattatgctg 241 ccacctccat ctacatgatc aacctggcag tctttgacct gctgctggtg ctctccctcc 301 cattcaagat ggtcctgtcc caggtacagt cccccttccc gtccctgtgc accctggtgg 361 agtgccttta cttcgtcagc atgtacggaa gcgtcttcac catctgcttc atcagcatgg 421 accggttctt ggccatccgt tacccgctac tggtgagcca cctccggtcc cccaggaaga 481 tctttgggat ctgctgcacc atctgggtcc tggtgtggac cggaagcatc cctatctaca 541 gtttccatgg gaaagtggaa aaatacatgt gcttccacaa catgtctgat gatacctgga 601 gcgccaaggt cttcttcccg ctggaggtgt ttggcttcct ccttcccatg ggcatcatgg 661 gcttcTGCTG CTCCAGGAGC ATCCACATCC TGCTGGGCCG CCGAGACCAC ACCCAGGACT 721 GGGTGCAGCA GAAAGCCTGC ATCTACAGCA TCGCAGCCAG CCTGGCTGTC TTCGTGGTCT 781 CCTTCCTCCC AGTCCACCTG GGGTTCTTCC TGCAGTTCCT GGTGAGAAAC AGCTTTATCG 841 TAGAGTGCAG AGCCAAGCAG AGCATCAGCT TCTTCTTGCA ATTGTCCATG TGTTTCTCCA 901 ACGTCAACTG CTGCCTGGAT GTTTTCTGCT ACTACTTTGT CATCAAAGAA TTCCGCATGA 961 ACATCAGGGC CCACCGGCCT TCCAGGGTCC AGCTGGTCCT GCAGGACACC ACGATCTCCC 1021 GGGGCGACCC AGCTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 1081 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 1141 CTTGGCTTTA TATATCTTGT GGAAAGGACG AATACACCAG GATCTAATTA AAAGTACGCG 1201 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt