Construct: ORF TRCN0000492152
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018353.1_s317c1
- Derived from:
- ccsbBroadEn_03261
- DNA Barcode:
- CCTATGACCTCGCAATGTAAAGAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MRPL27 (51264)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000492152
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 51264 | MRPL27 | mitochondrial ribosomal pro... | NM_016504.3 | 100% | 100% | |
| 2 | mouse | 94064 | Mrpl27 | mitochondrial ribosomal pro... | XM_006534567.2 | 60.3% | 59.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 510
- ORF length:
- 444
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc gtcggtggtg ttggcgctga ggacccggac agccgttaca tccttgcTAA 121 GCCCCACTCC GGCTACAGCT CTTGCTGTCA GATACGCATC CAAGAAGTCG GGTGGTAGCT 181 CCAAAAACCT CGGTGGAAAG TCATCAGGCA GACGCCAAGG CATTAAGAAA ATGGAAGGTC 241 ACTATGTTCA TGCTGGGAAC ATCATTGCAA CACAGCGCCA TTTCCGCTGG CACCCAGGTG 301 CCCATGTGGG TGTTGGGAAG AATAAATGTC TGTATGCCCT GGAAGAGGGG ATAGTCCGCT 361 ACACTAAGGA GGTCTACGTG CCTCATCCCA GAAACACGGA GGCTGTGGAT CTGATCACCA 421 GGCTGCCCAA GGGTGCTGTG CTCTACAAGA CTTTTGTCCA CGTGGTTCCT GCCAAGCCTG 481 AGGGCACCTT CAAACTGGTA GCTATGCTTT GCCCAACTTT CTTGTACAAA GTGGTTGATA 541 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 601 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGACCTAT 661 GACCTCGCAA TGTAAAGACA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 721 tgaaagatt