Construct: ORF TRCN0000492185
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001050.2_s317c1
- Derived from:
- ccsbBroadEn_12621
- DNA Barcode:
- TGACCACCCAAGCTAAACGAACCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- VASH2 (79805)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000492185
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 79805 | VASH2 | vasohibin 2 | NM_024749.5 | 49.8% | 42.3% | (many diffs) |
| 2 | human | 79805 | VASH2 | vasohibin 2 | NM_001301056.2 | 47.5% | 46.7% | (many diffs) |
| 3 | human | 79805 | VASH2 | vasohibin 2 | NM_001136474.3 | 29.2% | 28.4% | (many diffs) |
| 4 | human | 79805 | VASH2 | vasohibin 2 | NM_001136475.3 | 18.2% | 17.4% | (many diffs) |
| 5 | mouse | 226841 | Vash2 | vasohibin 2 | NM_144879.2 | 43.3% | 45.6% | (many diffs) |
| 6 | mouse | 226841 | Vash2 | vasohibin 2 | XM_006497149.4 | 38.9% | 41% | (many diffs) |
| 7 | mouse | 226841 | Vash2 | vasohibin 2 | XM_006497150.4 | 38.9% | 41% | (many diffs) |
| 8 | mouse | 226841 | Vash2 | vasohibin 2 | XM_011238913.3 | 38.9% | 41% | (many diffs) |
| 9 | mouse | 226841 | Vash2 | vasohibin 2 | NR_027352.1 | 11.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 573
- ORF length:
- 507
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgac cggctccgcg gccgacactc accgctgccc ccaccccaaa ggcgccaaag 121 gcacccggtC CCGGAGCAGC CACGCGCGGC CCGTGAGCCT CGCCACCAGC GGGGGCTCAG 181 AGGAGGAGGA CAAAGACGGC GGGGTGCTGT TCCACGTCAA CAAGAGCGGC TTCCCCATCG 241 ACAGCCACAC CTGGGAGCGC ATGTGGATGC ACGTGGCCAA GGTGCACCCT AAGGGGGGAG 301 AAATGGTGGG CGCCATCAGG AACGCCGCCT TCTTGGCAAA GCCTTCAATA CCCCAGGTCC 361 CAAACTACAG GCTGTCGATG ACGATCCCAG ACTGGCTCCA GGCGATCCAG AATTACATGA 421 AGACCCTACA ATATAATCAC ACAGGGACCC AGTTCTTTGA AATTAGGAAA ATGAGACCGC 481 TGAGTGGGTT AATGGAAACA GCAAAAGAAA TGACCCGAGA GTCCTTGCCT ATCAAATGCC 541 TTGAAGCTGT CATCCTGGGC ATAACCCACA GCTACCCAAC TTTCTTGTAC AAAGTGGTTG 601 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 661 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGATG 721 ACCACCCAAG CTAAACGAAC CAACGCGTTA AGTCgacaat caacctctgg attacaaaat 781 ttgtgaaaga tt