Construct: ORF TRCN0000492192
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021185.2_s317c1
- DNA Barcode:
- CGTCCGGCGAGATGATCTTTAAAA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- RRH (10692)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000492192
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10692 | RRH | retinal pigment epithelium-... | NM_006583.5 | 100% | 100% |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1083
- ORF length:
- 1011
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgctaaga aataatttag gcaacagttc agactctaaa aatgaagatg 121 gctcggtctt ttcacagact gaacacaata ttgttgcaac ttacttgatt atggcaggta 181 tgataagtat tatcagcaac ataatagttc tgggcatctt cattaagtac aaggaacttc 241 ggacacccac aaatgcaatt attattaacc tggctgttac tgatataggg gtcagtagca 301 ttggctatcc catgtctgct gcctcagatc tgtatggaag ttggaaattt ggatacgcag 361 gctgtcaggt ttatgctgga ttgaatattt tttttggaat ggcaagcatt ggattactca 421 cggtcgtggc tgtggaccga tacctgacca tctgccttcc tgacgtaggg agaagaatga 481 ccaccaacac ttacatcggc ttgattctgg gagcctggat caatggcctg ttttgggctt 541 tgatgcctat catagggtgg gctagttatg ccccagatcc tactggtgct acgtgtacca 601 taaactggag gaaaaatgat agatcttttg tgTCTTACAC CATGACAGTT ATTGCGATAA 661 ATTTTATTGT GCCCTTGACA GTGATGTTTT ACTGCTATTA CCATGTCACG CTATCCATTA 721 AACATCACAC TACCAGTGAC TGCACTGAGT CCCTCAACAG AGACTGGTCA GATCAGATAG 781 ATGTAACAAA GATGTCTGTG ATCATGATCT GCATGTTTCT GGTGGCATGG TCCCCTTATT 841 CCATCGTGTG CTTATGGGCT TCTTTTGGTG ACCCAAAGAA GATTCCTCCC CCCATGGCCA 901 TCATAGCTCC ACTGTTTGCA AAATCTTCTA CATTCTATAA CCCCTGCATT TATGTGGTTG 961 CTAATAAAAA GTTTCGGAGG GCAATGCTTG CCATGTTCAA ATGTCAGACT CACCAAACAA 1021 TGCCTGTGAC AAGTATTTTA CCCATGGATG TATCTCAAAA CCCATTGGCT TCTGGAAGAA 1081 TCTGAGACCC AGCTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 1141 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 1201 CTTGGCTTTA TATATCTTGT GGAAAGGACG ACGTCCGGCG AGATGATCTT TAAAAACGCG 1261 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt