Construct: ORF TRCN0000492205
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012572.1_s317c1
- Derived from:
- ccsbBroadEn_05091
- DNA Barcode:
- CGGGGATGGACGCTTCGGATGGTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TMEM74 (157753)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000492205
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 157753 | TMEM74 | transmembrane protein 74 | NM_153015.3 | 100% | 100% |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 981
- ORF length:
- 915
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga gctccactac cttgctaaga agagcaacca ggcagacctc tgtgatgcca 121 gggactggag ttcaagaggg ctgcctggtg accaggcaga tacagcagcc acaagagctg 181 ctctctgctg tcagaaacag tgtgcatcca ccccaagagc aaccgagatg gaagggtcta 241 aacttagttc ttctccagca tccccctcct cctctctgca aaacagtact cttcagccag 301 atgcctttcc accaggactt ctccactcag ggaacaacca aataacagcg gaacggaaag 361 tctgtaactg ctgcagccag gaattagaaa cttcttttac ctatgtggac aaaaacatca 421 acttggagca gcggaaccgg agctcgccat cagcaaaagg gcataatcac cctggggagc 481 ttggctggga aaatccaaat gagtggtccc aagaggctgc catatctttg atatctgaag 541 aggaggatga tacaagttca gaagccacgt cttcagggaa gtctatagac tatggtttca 601 tcagcgccat cttgttcttg gtcactggga tcctgctcgt gatcatctct tacatcgtcc 661 CACGGGAAGT GACTGTGGAC CCCAACACTG TGGCAGCCCG GGAGATGGAG CGCCTGGAGA 721 AGGAGAGTGC GAGGCTGGGG GCTCACCTGG ACCGCTGTGT GATTGCGGGG CTCTGCCTCC 781 TCACGCTGGG GGGCGTCATC CTGTCCTGCT TGTTAATGAT GTCCATGTGG AAGGGGGAGC 841 TCTATCGTCG AAACAGATTT GCCTCTTCCA AAGAGTCTGC AAAACTCTAT GGTTCTTTCA 901 ACTTCAGGAT GAAAACCAGC ACGAATGAAA ACACTCTGGA ACTGTCCTTG GTAGAGGAAG 961 ATGCGCTTGC TGTACAGAGT TACCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 1021 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 1081 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGACGGG GATGGACGCT 1141 TCGGATGGTC ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt