Construct: ORF TRCN0000492243
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021256.2_s317c1
- DNA Barcode:
- TCCCGATGAAGAAATTCCCTAGAA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- CETP (1071)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000492243
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1071 | CETP | cholesteryl ester transfer ... | NM_000078.3 | 99.9% | 99.7% | 1264G>A |
| 2 | human | 1071 | CETP | cholesteryl ester transfer ... | NM_001286085.2 | 87.7% | 87.6% | 746_747ins180;1084G>A |
| 3 | human | 1071 | CETP | cholesteryl ester transfer ... | XM_006721124.3 | 52.3% | 51.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1551
- ORF length:
- 1479
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgctggct gccacagtcc tgaccctggc cctgctgggc aatgcccatg 121 cctgctccaa aggcacctcg cacgaggcag gcatcgtgtg ccgcatcacc aagcctgccc 181 tcctggtgtt gaaccacgag actgccaagg tgatccagac cgccttccag cgagccagct 241 acccagatat cacgggcgag aaggccatga tgctccttgg ccaagtcaag tatgggttgc 301 acaacatcca gatcagccac ttgtccatcg ccagcagcca ggtggagctg gtggaagcca 361 agtccattga tgtctccatt cagaacgtgt ctgtggtctt caaggggacc ctgaagtatg 421 gctacaccac tgcctggtgg ctgggtattg atcagtccat tgacttcgag atcgactctg 481 ccattgacct ccagatcaac acacagctga cctgtgactc tggtagagtg cggaccgatg 541 cccctgactg ctacctgtct ttccataagc tgctcctgca tctccaaggg gagcgagagc 601 ctgggtggat caagcagctg ttcacaaatt tcatctcctt caccctgaag ctggtcctga 661 agggacagat ctgcaaagag atcaacgtca tctctaacat catggccgat tttgtccaga 721 caagggctgc cagcatcctt tcagatggag acattggggt ggacatttcc ctgacaggtg 781 atcccgtcat cacagcctcc tacctggagt cccatcacaa gggtcatttc atctacaaga 841 atgtctcaga ggacctcccc ctccccacct tctcgcccac actgctgggg gactcccgca 901 tgctgtactt ctggttctct gagcgagtct tccactcgct ggccaaggta gctttccagg 961 atggccgcct catgctcagc ctgatgggag acgagttcaa ggcagtgctg gagacctggg 1021 gcttcaacac caaccaggaa atcttccaag aggttgtcgg cggcttcccc agccaggccc 1081 aagtcaccgt ccactgcctc aagatgccca agatctcctg ccaaaacaag ggagtcgtgg 1141 tcaattcttc agtgatggtg aaatTCCTCT TTCCACGCCC AGACCAGCAA CATTCTGTAG 1201 CTTACACATT TGAAGAGGAT ATCGTGACTA CCGTCCAGGC CTCCTATTCT AAGAAAAAGC 1261 TCTTCTTAAG CCTCTTGGAT TTCCAGATTA CACCAAAGAC TGTTTCCAAC TTGACTGAGA 1321 GCAGCTCCGA GTCCATCCAG AGCTTCCTGC AGTCAATGAT CACCGCTGTG GGCATCCCTG 1381 AGGTCATGTC TCGGCTCGAG GTAGTGTTTA CAGCCCTCAT GAACAGCAAA GGCGTGAGCC 1441 TCTTCGACAT CATCAACCCT GAGATTATCA CTCGAGATGG CTTCCTGCTG CTGCAGATGG 1501 ACTTTGGCTT CCCTGAGCAC CTGCTGGTGG ATTTCCTCCA GAGCTTGAGC TAGAACCCAG 1561 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 1621 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 1681 TATCTTGTGG AAAGGACGAT CCCGATGAAG AAATTCCCTA GAAACGCGTT AAGTCgacaa 1741 tcaacctctg gattacaaaa tttgtgaaag att