Construct: ORF TRCN0000492254

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF019591.2_s317c1
DNA Barcode:
ACCATATCGACGACGTTATGACGT
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
PFKM (5213)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000492254
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 5213 PFKM phosphofructokinase, muscle NM_000289.6 99.9% 99.8% 2340_2341insG
2 human 5213 PFKM phosphofructokinase, muscle NM_001166687.1 99.9% 99.8% 2340_2341insG
3 human 5213 PFKM phosphofructokinase, muscle NM_001166688.1 99.9% 99.8% 2340_2341insG
4 human 5213 PFKM phosphofructokinase, muscle NM_001354742.1 99.9% 99.8% 2340_2341insG
5 human 5213 PFKM phosphofructokinase, muscle NM_001354743.1 99.9% 99.8% 2340_2341insG
6 human 5213 PFKM phosphofructokinase, muscle NM_001354744.1 99.9% 99.8% 2340_2341insG
7 human 5213 PFKM phosphofructokinase, muscle XM_024449022.1 99.9% 99.8% 2340_2341insG
8 human 5213 PFKM phosphofructokinase, muscle NM_001354741.1 98.9% 98.8% 1_24del;2364_2365insG
9 human 5213 PFKM phosphofructokinase, muscle NM_001354745.1 96.2% 96.1% 0_1ins87;2253_2254insG
10 human 5213 PFKM phosphofructokinase, muscle NM_001363619.1 95.9% 95.9% 843_844ins93;2247_2248insG
11 human 5213 PFKM phosphofructokinase, muscle XM_024449021.1 95.9% 95.8% 1_99del;2439_2440insG
12 human 5213 PFKM phosphofructokinase, muscle NM_001354746.1 94.5% 94.4% 934_935ins126;2214_2215insG
13 human 5213 PFKM phosphofructokinase, muscle NM_001354740.1 94.1% 94% 1_144del;2484_2485insG
14 human 5213 PFKM phosphofructokinase, muscle NM_001354747.1 93.4% 93% (many diffs)
15 human 5213 PFKM phosphofructokinase, muscle NM_001354748.1 93.4% 93% (many diffs)
16 human 5213 PFKM phosphofructokinase, muscle NM_001166686.2 91.6% 91.5% 1_213del;2553_2554insG
17 human 5213 PFKM phosphofructokinase, muscle NM_001354737.1 91.6% 91.5% 1_213del;2553_2554insG
18 human 5213 PFKM phosphofructokinase, muscle NM_001354738.1 91.6% 91.5% 1_213del;2553_2554insG
19 human 5213 PFKM phosphofructokinase, muscle NM_001354739.1 91.6% 91.5% 1_213del;2553_2554insG
20 human 5213 PFKM phosphofructokinase, muscle XM_005268979.1 91.6% 91.5% 1_213del;2553_2554insG
21 human 5213 PFKM phosphofructokinase, muscle XM_024449020.1 91.2% 91.2% 1_222del;2562_2563insG
22 human 5213 PFKM phosphofructokinase, muscle NM_001354735.1 88.3% 88.2% 1_309del;2649_2650insG
23 human 5213 PFKM phosphofructokinase, muscle NM_001354736.1 88.3% 88.2% 1_309del;2649_2650insG
24 human 5213 PFKM phosphofructokinase, muscle XM_005268974.1 88.3% 88.2% 1_309del;2649_2650insG
25 human 5213 PFKM phosphofructokinase, muscle XM_005268976.3 88.3% 88.2% 1_309del;2649_2650insG
26 human 5213 PFKM phosphofructokinase, muscle XM_017019469.1 87.9% 87.9% 1_213del;1056_1057ins93;2460_2461insG
27 human 5213 PFKM phosphofructokinase, muscle XM_011538487.1 84.7% 84.7% 1_309del;1152_1153ins93;2556_2557insG
28 human 5213 PFKM phosphofructokinase, muscle NR_148958.1 69.2% 1_185del;1312_1466del;2681_3379delinsG
29 human 5213 PFKM phosphofructokinase, muscle NR_148954.1 67.3% 1_185del;1029_1280del;2778_3476delinsG
30 human 5213 PFKM phosphofructokinase, muscle NR_148959.1 67% (many diffs)
31 human 5213 PFKM phosphofructokinase, muscle NR_148956.1 65.1% (many diffs)
32 human 5213 PFKM phosphofructokinase, muscle NR_148957.1 64.4% (many diffs)
33 human 5213 PFKM phosphofructokinase, muscle NR_148955.1 56.9% (many diffs)
34 mouse 18642 Pfkm phosphofructokinase, muscle NM_001163487.1 90.1% 97.6% (many diffs)
35 mouse 18642 Pfkm phosphofructokinase, muscle NM_001163488.1 90.1% 97.6% (many diffs)
36 mouse 18642 Pfkm phosphofructokinase, muscle NM_021514.4 90.1% 97.6% (many diffs)
37 mouse 18642 Pfkm phosphofructokinase, muscle XM_006520602.2 90.1% 97.6% (many diffs)
38 mouse 18642 Pfkm phosphofructokinase, muscle XM_006520601.3 82.7% 89.6% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
2409
ORF length:
2343
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaggat
61ccaccatgac ccatgaagag caccatgcag ccaaaaccct ggggattggc aaagccattg
121ctgtcttaac ctctggtgga gatgcccaag gtatgaatgc tgctgtcagg gctgtggttc
181gagttggtat cttcaccggt gcccgtgtct tctttgtcca tgagggttat caaggcctgg
241tggatggtgg agatcacatc aaggaagcca cctgggagag cgtttcgatg atgcttcagc
301tgggaggcac ggtgattgga agtgcccggt gcaaggactt tcgggaacga gaaggacgac
361tccgagctgc ctacaacctg gtgaagcgtg ggatcaccaa tctctgtgtc attgggggtg
421atggcagcct cactggggct gacaccttcc gttctgagtg gagtgacttg ttgagtgacc
481tccagaaagc aggtaagatc acagatgagg aggctacgaa gtccagctac ctgaacattg
541tgggcctggt tgggtcaatt gacaatgact tctgtggcac cgatatgacc attggcactg
601actctgccct gcatcggatc atggaaattg tagatgccat cactaccact gcccagagcc
661accagaggac atttgtgtta gaagtaatgg gccgccactg tggatacctg gcccttgtca
721cctctctgtc ctgtggggcc gactgggttt ttattcctga atgtccacca gatgacgact
781gggaggaaca cctttgtcgc cgactcagcg agacaaggac ccgtggttct cgtctcaaca
841tcatcattgt ggctgagggt gcaattgaca agaatggaaa accaatcacc tcagaagaca
901tcaagaatct ggtggttaag cgtctgggat atgacacccg ggttactgtc ttggggcatg
961tgcagagggg tgggacgcca tcagcctttg acagaattct gggcagcagg atgggtgtgg
1021aagcagtgat ggcacttttg gaggggaccc cagatacccc agcctgtgta gtgagcctct
1081ctggtaacca ggctgtgcgc ctgcccctca tggaatgtgt ccaggtgacc aaagatgtga
1141ccaaggccat ggatgagaag aaatttgacg aagccctgaa gctgagaggc cggagcttca
1201tgaacaactg ggaggtgtac aagcttctag ctcatgtcag acccccggta tctaagagtg
1261gttcgcacac agtggctgtg atgaacgtgg gggctccggc tgcaggcatg aatgctgctg
1321ttcgctccac tgtgaggatt ggccttatcc agggcaaccg agtgctcgtt gtccatgatg
1381gtttcgaggg cctggccaag gggcagatag aggaagctgg ctggagctat gttgggggct
1441ggactggcca aggtggctct aaacttggga ctaaaaggac tctacccaag aagagctttg
1501aacagatcag tgccaatata actaagttta acattcaggg ccttgtcatc attgggggct
1561ttgaggctta cacagggggc ctggaactga tggagggcag gaagcagttt gatgagctct
1621gcatcccatt tgtggtcatt cctgctacag tctccaacaa tgtccctggc tcagacttca
1681gcgttggggc tgacacagca ctcaatacta tctgcacaac ctgtgaccgc atcaagcagt
1741cagcagctgg caccaagcgt cgggtgttta tcattgagac tatgggtggc tactgtggct
1801acctggctac catggctgga ctggcagctg gggccgatgc tgcctacatt tttgaggagc
1861ccttcaccat tcgagacctg caggcaaatg ttgaacatct ggtgcaaaag atgaaaacaa
1921ctgtgaaaag gggcttggtg ttaaggaatg aaaagtgcaa tgagaactat accactgact
1981tcattttcaa cctgtactct gaggagggga agggcatctt cgacagcagg aagaatgtgc
2041ttggtcacat gcagcagggt gggagcccaa ccccatttga taggaatttt gccactaaga
2101tgggcgccaa ggctatgaac tggatgtctg ggaaaatcaa agagagttac cgtaatgggc
2161ggatctttgc caatactcca gattcgggct gtgttctggg gatgcgtaag agggctctgg
2221tcttccaacc agtggcTGAG CTGAAGGACC AGACAGATTT TGAGCATCGA ATCCCCAAGG
2281AACAGTGGTG GCTGAAACTG AGGCCCATCC TCAAAATCCT AGCCAAGTAC GAGATTGACT
2341TGGACACTTC AGACCATGCC CACCTGGAGC ACATCACCCG GAAGCGGTCC GGGGAAGCTG
2401CCGTCGACCC AGCTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC
2461TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT
2521CTTGGCTTTA TATATCTTGT GGAAAGGACG AACCATATCG ACGACGTTAT GACGTACGCG
2581TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt

Download FASTA (ORF) (Full)