Construct: ORF TRCN0000492344

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF020871.2_s317c1
DNA Barcode:
CTGCGGTTTGCCTATATCCCCGTT
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Has stop codon in insert

Originally Annotated References:

Gene:
CHRM5 (1133)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000492344
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 1133 CHRM5 cholinergic receptor muscar... NM_001320917.2 100% 100%
2 human 1133 CHRM5 cholinergic receptor muscar... NM_012125.4 100% 100%
3 mouse 213788 Chrm5 cholinergic receptor, musca... NM_205783.2 88.2% 88.7% (many diffs)
4 mouse 213788 Chrm5 cholinergic receptor, musca... XM_006499099.3 88.2% 88.7% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
72
ORF end:
1668
ORF length:
1596
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61caggcttcac catggaaggg gattcttacc acaatgcaac caccgtcaat ggcaccccag
121taaatcacca gcctttggaa cgccacaggt tgtgggaagt catcaccatt gcagctgtga
181ctgctgtggt aagcctgatc accattgtgg gcaatgtctt ggtcatgatc tccttcaaag
241tcaacagcca gctcaagaca gttaacaact attacctgct cagcttagcc tgtgcagatc
301tcatcattgg aatcttctcc atgaacctct acaccaccta catcctcatg ggacgctggg
361ctctcgggag tctggcttgt gacctttggc ttgcactgga ctacgtggcc agcaacgctt
421ctgtcatgaa ccttctggtg atcagttttg accgttactt ttccatcaca agacccttga
481catatcgggc caagcgtact ccgaaaaggg ctggcatcat gattggcttg gcctggctga
541tctccttcat cctctgggcc ccagcaatcc tctgctggca gtacttggtt gggaagcgga
601cagttccact ggatgagtgc cagatccagt ttctctctga gcccaccatc acttttggca
661ctgccattgc tgccttctac atccctgttt ctgtcatgac catcctctac tgtcgaatct
721accgggaaac agagaagcga accaaggacc tggctgacct ccagggttct gactctgtga
781ccaaagctga gaagagaaag ccagctcata gggctctgtt cagatcctgc ttgcgctgtc
841ctcgacccac cctggcccag cgggaaagga accaggcctc ctggtcatcc tcccgcagga
901gcacctccac cactgggaag ccatcccaag ccactggccc aagcgccaat tgggccaaag
961ctgagcagct caccacctgt agcagctacc cttcctcaga ggatgaggac aagcccgcca
1021ctgaccctgt cctccaagtg gtctacaaga gtcagggtaa ggaaagccca ggggaagaat
1081tcagtgctga agagactgag gaaacttttg tgaaagctga aactgaaaaa agtgactatg
1141acaccccaaa ctaccttctg tctccagcag ctgcTCATAG ACCCAAGAGT CAGAAATGTG
1201TGGCCTATAA GTTCCGATTG GTGGTAAAAG CTGACGGGAA CCAGGAGACC AACAATGGCT
1261GTCACAAGGT GAAAATCATG CCCTGCCCCT TCCCAGTGGC CAAGGAACCT TCAACGAAAG
1321GCCTCAATCC CAACCCCAGC CATCAAATGA CCAAACGAAA GAGAGTGGTC CTAGTCAAAG
1381AGAGGAAAGC AGCCCAGACA CTGAGTGCCA TTCTCCTGGC CTTCATCATC ACATGGACCC
1441CGTATAACAT CATGGTCCTG GTTTCTACCT TCTGTGACAA GTGTGTCCCA GTCACCCTGT
1501GGCACTTGGG CTATTGGTTG TGCTATGTCA ATAGCACTGT CAACCCCATC TGCTATGCCC
1561TCTGCAACAG AACCTTCAGG AAGACCTTTA AGATGCTGCT TCTCTGCCGA TGGAAAAAGA
1621AAAAAGTGGA AGAGAAGTTG TACTGGCAGG GGAACAGCAA GCTACCCTAG GACCCAGCTT
1681TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT
1741CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT
1801CTTGTGGAAA GGACGACTGC GGTTTGCCTA TATCCCCGTT ACGCGTTAAG TCgacaatca
1861acctctggat tacaaaattt gtgaaagatt

Download FASTA (ORF) (Full)

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