Construct: ORF TRCN0000492344
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020871.2_s317c1
- DNA Barcode:
- CTGCGGTTTGCCTATATCCCCGTT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- CHRM5 (1133)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000492344
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1133 | CHRM5 | cholinergic receptor muscar... | NM_001320917.2 | 100% | 100% | |
| 2 | human | 1133 | CHRM5 | cholinergic receptor muscar... | NM_012125.4 | 100% | 100% | |
| 3 | mouse | 213788 | Chrm5 | cholinergic receptor, musca... | NM_205783.2 | 88.2% | 88.7% | (many diffs) |
| 4 | mouse | 213788 | Chrm5 | cholinergic receptor, musca... | XM_006499099.3 | 88.2% | 88.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1668
- ORF length:
- 1596
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggaaggg gattcttacc acaatgcaac caccgtcaat ggcaccccag 121 taaatcacca gcctttggaa cgccacaggt tgtgggaagt catcaccatt gcagctgtga 181 ctgctgtggt aagcctgatc accattgtgg gcaatgtctt ggtcatgatc tccttcaaag 241 tcaacagcca gctcaagaca gttaacaact attacctgct cagcttagcc tgtgcagatc 301 tcatcattgg aatcttctcc atgaacctct acaccaccta catcctcatg ggacgctggg 361 ctctcgggag tctggcttgt gacctttggc ttgcactgga ctacgtggcc agcaacgctt 421 ctgtcatgaa ccttctggtg atcagttttg accgttactt ttccatcaca agacccttga 481 catatcgggc caagcgtact ccgaaaaggg ctggcatcat gattggcttg gcctggctga 541 tctccttcat cctctgggcc ccagcaatcc tctgctggca gtacttggtt gggaagcgga 601 cagttccact ggatgagtgc cagatccagt ttctctctga gcccaccatc acttttggca 661 ctgccattgc tgccttctac atccctgttt ctgtcatgac catcctctac tgtcgaatct 721 accgggaaac agagaagcga accaaggacc tggctgacct ccagggttct gactctgtga 781 ccaaagctga gaagagaaag ccagctcata gggctctgtt cagatcctgc ttgcgctgtc 841 ctcgacccac cctggcccag cgggaaagga accaggcctc ctggtcatcc tcccgcagga 901 gcacctccac cactgggaag ccatcccaag ccactggccc aagcgccaat tgggccaaag 961 ctgagcagct caccacctgt agcagctacc cttcctcaga ggatgaggac aagcccgcca 1021 ctgaccctgt cctccaagtg gtctacaaga gtcagggtaa ggaaagccca ggggaagaat 1081 tcagtgctga agagactgag gaaacttttg tgaaagctga aactgaaaaa agtgactatg 1141 acaccccaaa ctaccttctg tctccagcag ctgcTCATAG ACCCAAGAGT CAGAAATGTG 1201 TGGCCTATAA GTTCCGATTG GTGGTAAAAG CTGACGGGAA CCAGGAGACC AACAATGGCT 1261 GTCACAAGGT GAAAATCATG CCCTGCCCCT TCCCAGTGGC CAAGGAACCT TCAACGAAAG 1321 GCCTCAATCC CAACCCCAGC CATCAAATGA CCAAACGAAA GAGAGTGGTC CTAGTCAAAG 1381 AGAGGAAAGC AGCCCAGACA CTGAGTGCCA TTCTCCTGGC CTTCATCATC ACATGGACCC 1441 CGTATAACAT CATGGTCCTG GTTTCTACCT TCTGTGACAA GTGTGTCCCA GTCACCCTGT 1501 GGCACTTGGG CTATTGGTTG TGCTATGTCA ATAGCACTGT CAACCCCATC TGCTATGCCC 1561 TCTGCAACAG AACCTTCAGG AAGACCTTTA AGATGCTGCT TCTCTGCCGA TGGAAAAAGA 1621 AAAAAGTGGA AGAGAAGTTG TACTGGCAGG GGAACAGCAA GCTACCCTAG GACCCAGCTT 1681 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 1741 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 1801 CTTGTGGAAA GGACGACTGC GGTTTGCCTA TATCCCCGTT ACGCGTTAAG TCgacaatca 1861 acctctggat tacaaaattt gtgaaagatt