Construct: ORF TRCN0000492357
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020447.2_s317c1
- DNA Barcode:
- TCGTAACGCCAGGTATCTCGGGTA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- PCK1 (5105)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000492357
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5105 | PCK1 | phosphoenolpyruvate carboxy... | NM_002591.4 | 99.8% | 99.8% | 550G>C;1140T>C |
| 2 | human | 5105 | PCK1 | phosphoenolpyruvate carboxy... | XM_024451888.1 | 78.3% | 78.1% | (many diffs) |
| 3 | mouse | 18534 | Pck1 | phosphoenolpyruvate carboxy... | NM_011044.2 | 85.5% | 90.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1938
- ORF length:
- 1866
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgcctcct cagctgcaaa acggcctgaa cctctcggcc aaagttgtcc 121 agggaagcct ggacagccta ccccaggcag tgagggagtt tctcgagaat aacgctgagc 181 tgtgtcagcc tgatcacatc cacatctgtg acggctctga ggaggagaat gggcggcttc 241 tgggccagat ggaggaagag ggcatcctca ggcggctgaa gaagtatgac aactgctggt 301 tggctctcac tgaccccagg gatgtggcca ggatcgaaag caagacggtt atcgtcaccc 361 aagagcaaag agacacagtg cccatcccca aaacaggcct cagccagctc ggtcgctgga 421 tgtcagagga ggattttgag aaagcgttca atgccaggtt cccagggtgc atgaaaggtc 481 gcaccatgta cgtcatccca ttcagcatgg ggccgctggg ctcgcctctg tcaaagatcg 541 gcatcgagct gacggattca ccctacgtgg tggccagcat gcggatcatg acgcggatgg 601 gcacgcccgt cctggaagca ctgggcgatg gggagtttgt caaatgcctc cattctgtgg 661 ggtgccctct gcctttacaa aagcctttgg tcaacaactg gccctgcaac ccggagctga 721 cgctcatcgc ccacctgcct gaccgcagag agatcatctc ctttggcagt gggtacggcg 781 ggaactcgct gctcgggaag aagtgctttg ctctcaggat ggccagccgg ctggccaagg 841 aggaagggtg gctggcagag cacatgctga ttctgggtat aaccaaccct gagggtgaga 901 agaagtacct ggcggccgca tttcccagcg cctgcgggaa gaccaacctg gccatgatga 961 accccagcct ccccgggtgg aaggttgagt gcgtcgggga tgacattgcc tggatgaagt 1021 ttgacgcaca aggtcattta agggccatca acccagaaaa tggctttttc ggtgtcgctc 1081 ctgggacttc agtgaagacc aaccccaatg ccatcaagac catccagaag aacacaatct 1141 ttaccaatgt ggccgagacc agcgacgggg gcgtttactg ggaaggcatt gatgagccgc 1201 tagcttcagg cgtcaccatc acgtcctgga agaataagga gtggagctca gaggatgggg 1261 aaccttgtgc ccaccccaac tcgaggttct gcacccctgc cagccagtgc cccatcattg 1321 atgctgcctg ggagtctccg gaaggtgttc ccattgaagg cattatcttt ggaggccgta 1381 gacctgctgg tgtccctcta gtctatgaag ctctcagctg gcaacatgga gtctttgtgg 1441 gggcggccat gagatcagag gccacagcgg ctgcagaaca taaaggcaaa atcatcatgc 1501 atgacccctt tgccatgcgg cccttctttg gctacaacTT CGGCAAATAC CTGGCCCACT 1561 GGCTTAGCAT GGCCCAGCAC CCAGCAGCCA AACTGCCCAA GATCTTCCAT GTCAACTGGT 1621 TCCGGAAGGA CAAGGAAGGC AAATTCCTCT GGCCAGGCTT TGGAGAGAAC TCCAGGGTGC 1681 TGGAGTGGAT GTTCAACCGG ATCGATGGAA AAGCCAGCAC CAAGCTCACG CCCATAGGCT 1741 ACATCCCCAA GGAGGATGCC CTGAACCTGA AAGGCCTGGG GCACATCAAC ATGATGGAGC 1801 TTTTCAGCAT CTCCAAGGAA TTCTGGGAGA AGGAGGTGGA AGACATCGAG AAGTATCTGG 1861 AGGATCAAGT CAATGCCGAC CTCCCCTGTG AAATCGAGAG AGAGATCCTT GCCTTGAAGC 1921 AAAGAATAAG CCAGATGTAG GACCCAGCTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 1981 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 2041 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGATCGT AACGCCAGGT 2101 ATCTCGGGTA ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt