Construct: shRNA TRCN0000559394

Construct Description:

Construct Type:
shRNA
Other Identifiers:
n/a
DNA Barcode:
ACAACAGCCACAACGTCTATA

Original Target:

Taxon:
Control
Gene:
GFP (GFP)
Gene Description:
clonetechGfp
Transcript:
Broad clonetechGfp.1 (CURRENT)
Match location:
Position 437 (CDS)

Vector Information:

Vector Backbone:
pLKO_047
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
2A-eGFP
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000559394)
Selection Marker:
PuroR
Visible Reporter:
eGFP

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACAACAGCCACAACGTCTATA
Hairpin Sequence:
5'-CCGG-ACAACAGCCACAACGTCTATA-CTCGAG-TATAGACGTTGTGGCTGTTGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACAACAGCCACAACGTCTATACTCGAGTATAGACGTTGTGGCTGTTGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACAACAGCCACAACGTCTATACTCGAGTATAGACGTTGTGGCTGTTGT-3'

Other clones with same target sequence:

  1. TRCN0000072181
  2. TRCN0000231753
  3. TRCN0000559393
  4. TRCN0000559397
  5. TRCN0000559399
  6. TRCN0000559403
  7. TRCN0000559407
  8. TRCN0000559396
  9. TRCN0000559401
  10. TRCN0000559408