Construct: ORF ccsbBroad304_00027
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017530.1_s304c1, BRDN0000405196
- Derived from:
- ccsbBroadEn_00027
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ADH1A (124)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_00027
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 124 | ADH1A | alcohol dehydrogenase 1A (c... | NM_000667.4 | 100% | 100% | |
| 2 | human | 125 | ADH1B | alcohol dehydrogenase 1B (c... | NM_000668.6 | 94.9% | 93.6% | (many diffs) |
| 3 | human | 126 | ADH1C | alcohol dehydrogenase 1C (c... | NM_000669.5 | 93.8% | 92.8% | (many diffs) |
| 4 | human | 125 | ADH1B | alcohol dehydrogenase 1B (c... | NM_001286650.2 | 84.7% | 83.7% | (many diffs) |
| 5 | human | 126 | ADH1C | alcohol dehydrogenase 1C (c... | NR_133005.2 | 69.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1194
- ORF length:
- 1125
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat gagcacagca ggaaaagtaa tcaaatgcaa agcagctgtg ctatgggagt 121 taaagaaacc cttttccatt gaggaggtgg aggttgcacc tcctaaggcc catgaagttc 181 gtattaagat ggtggctgta ggaatctgtg gcacagatga ccacgtggtt agtggtacca 241 tggtgacccc acttcctgtg attttaggcc atgaggcagc cggcatcgtg gagagtgttg 301 gagaaggggt gactacagtc aaaccaggtg ataaagtcat cccactcgct attcctcagt 361 gtggaaaatg cagaatttgt aaaaacccgg agagcaacta ctgcttgaaa aacgatgtaa 421 gcaatcctca ggggaccctg caggatggca ccagcaggtt cacctgcagg aggaagccca 481 tccaccactt ccttggcatc agcaccttct cacagtacac agtggtggat gaaaatgcag 541 tagccaaaat tgatgcagcc tcgcctctag agaaagtctg tctcattggc tgtggatttt 601 caactggtta tgggtctgca gtcaatgttg ccaaggtcac cccaggctct acctgtgctg 661 tgtttggcct gggaggggtc ggcctatctg ctattatggg ctgtaaagca gctggggcag 721 ccagaatcat tgcggtggac atcaacaagg acaaatttgc aaaggccaaa gagttgggtg 781 ccactgaatg catcaaccct caagactaca agaaacccat ccaggaggtg ctaaaggaaa 841 tgactgatgg aggtgtggat ttttcatttg aagtcatcgg tcggcttgac accatgatgg 901 cttccctgtt atgttgtcat gaggcatgtg gcacaagtgt catcgtaggg gtacctcctg 961 attcccaaaa cctctcaatg aaccctatgc tgctactgac tggacgtacc tggaagggag 1021 ctattcttgg tggctttaaa agtaaagaat gtgtcccaaa acttgtggct gattttatgg 1081 ctaagaagtt ttcattggat gcattaataa cccatgtttt accttttgaa aaaataaatg 1141 aaggatttga cctgcttcac tctgggaaaa gtatccgtac cattctgatg tttttgccaa 1201 ctttcttgta caaagtggtt ggtaagccta tccctaaccc tctcctcggt ctcgattcta 1261 cgtagtaatg agctagcgct aaccggtggc gcgttaagtc gacaatcaac ctctggatta