Transcript: Human NM_000669.5

Homo sapiens alcohol dehydrogenase 1C (class I), gamma polypeptide (ADH1C), transcript variant 1, mRNA.

Source:
NCBI, updated 2019-09-19
Taxon:
Homo sapiens (human)
Gene:
ADH1C (126)
Length:
1454
CDS:
72..1199

Additional Resources:

NCBI RefSeq record:
NM_000669.5
NBCI Gene record:
ADH1C (126)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_000669.5, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000221857 GCACGTGGAAAGGAGCTATTT pLKO.1 1009 CDS 100% 13.200 10.560 N ADH1C n/a
2 TRCN0000221860 GCTTCGCTCTGGAAAGAGTAT pLKO.1 1157 CDS 100% 4.950 3.960 N ADH1C n/a
3 TRCN0000419086 CCAAACTTGTGGCTGACTTTA pLKO_005 1060 CDS 100% 13.200 9.240 N ADH1C n/a
4 TRCN0000221858 CGTTCAGATGAGCATGTGGTT pLKO.1 213 CDS 100% 2.640 1.848 N ADH1C n/a
5 TRCN0000436454 GATTCCCAGAACCTCTCAATA pLKO_005 963 CDS 100% 13.200 7.920 N ADH1C n/a
6 TRCN0000413586 GGTCGGCCTATCTGTTGTTAT pLKO_005 680 CDS 100% 13.200 7.920 N ADH1C n/a
7 TRCN0000412772 GACATCAACAAGGACAAATTT pLKO_005 741 CDS 100% 15.000 7.500 Y ADH1A n/a
8 TRCN0000221875 GCTTCCCTGTTATGTTGTCAT pLKO.1 903 CDS 100% 4.950 2.475 Y ADH1B n/a
9 TRCN0000221872 CGCATTAAGATGGTGGCTGTA pLKO.1 183 CDS 100% 4.050 2.025 Y ADH1B n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_000669.5, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_05777 pDONR223 100% 99.9% 100% None 453A>G n/a
2 ccsbBroad304_05777 pLX_304 0% 99.9% 100% V5 453A>G n/a
3 TRCN0000480425 AAGTATATATCCCGTTTTGCATTT pLX_317 34.5% 99.9% 100% V5 453A>G n/a
4 ccsbBroadEn_05776 pDONR223 100% 95.5% 94.4% None (many diffs) n/a
5 ccsbBroad304_05776 pLX_304 0% 95.5% 94.4% V5 (many diffs) n/a
6 ccsbBroadEn_00027 pDONR223 100% 93.8% 92.8% None (many diffs) n/a
7 ccsbBroad304_00027 pLX_304 0% 93.8% 92.8% V5 (many diffs) n/a
8 TRCN0000479161 CAAACCAATCCTGCATCCGGACTC pLX_317 44.9% 93.8% 92.8% V5 (many diffs) n/a
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