Construct: ORF ccsbBroad304_00046
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017112.1_s304c1, BRDN0000403251
- Derived from:
- ccsbBroadEn_00046
- DNA Barcode:
- None
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- AKT1 (207)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_00046
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 207 | AKT1 | AKT serine/threonine kinase 1 | NM_001014431.2 | 79.2% | 57.1% | (many diffs) |
| 2 | human | 207 | AKT1 | AKT serine/threonine kinase 1 | NM_001014432.1 | 79.2% | 57.1% | (many diffs) |
| 3 | human | 207 | AKT1 | AKT serine/threonine kinase 1 | NM_005163.2 | 79.2% | 57.1% | (many diffs) |
| 4 | human | 207 | AKT1 | AKT serine/threonine kinase 1 | XR_002957536.1 | 27.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1041
- ORF length:
- 975
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgag cgacgtggct attgtgaagg agggttggct gcacaaacga ggggagtaca 121 tcaagacctg gcggccacgc tacttcctcc tcaagaatga tggcaccttc attggctaca 181 aggagcggcc gcaggatgtg gaccaacgtg aggctcccct caacaacttc tctgtggcgc 241 agtgccagct gatgaagacg gagcggcccc ggcccaacac cttcatcatc cgctgcctgc 301 agtggaccac tgtcatcgaa cgcaccttcc atgtggagac tcctgaggag cgggaggagt 361 ggacaaccgc catccagact gtggctgacg gcctcaagaa gcaggaggag gaggagatgg 421 acttccggtc gggctcaccc agtgacaact caggggctga agagatggag gtgtccctgg 481 ccaagcccaa gcaccgcgtg accatgaacg agtttgagta cctgaagctg ctgggcaagg 541 gcactttcgg caaggtgatc ctggtgaagg agaaggccac aggccgctac tacgccatga 601 agatcctcaa gaaggaagtc atcgtggcca aggacgaggt ggcccacaca ctcaccgaga 661 accgcgtcct gcagaactcc aggcacccct tcctcacagc cctgaagtac tctttccaga 721 cccacgaccg cctctgcttt gtcatggagt acgccaacgg gggcgagctg ttcttccacc 781 tgtcccggga gcgtgtgttc tccgaggacc gggcccgctt ctatggcgct gagattgtgt 841 cagcGATGGG CTCACTGTCT CGGAAATCGA ACATTGTGCA GCGAGTCCTG AGCTGGGATA 901 ATCCTCAAGG TGCTCCAAGA CGGACCTCGT CACATCAAAG GATTCCCTGC TGTGATAGGA 961 TGGGATCAAG GGACGGTCCC GCAAGAAGAA CGTTTGCTGC CCTCTCCAGG AGCCGGTCTG 1021 CCTTGTTCCT GTATGGAGAA TAATTACGAC GTTCCAGGAA TCCTGGGGGG GGACTTGGCA 1081 GTGTTCCCCT ACGACAAAAA TGCGCGTGTC GCCTAAGCCT CCAACAACCA GTACTAAGGA 1141 GATGCTTTTT TAGGGGGCAG GGGAGTTGGG GGAATCTACT TACGGGATGG CAAAGGGAAC 1201 TCAGGGCTAG TCCTTATTTT GAGCACTGCT ATAGATTCCC ACTAAGCTGA GCGTAATTAG 1261 GGCTCTCAAG ATCCCAAGGG AGTCAAGTTC CTCGCTGTTT TGCAGGATTA GTGTGGCGAA 1321 AAGTTAACGA GAAGAAGCTC AGTCCGCCCT GCGAGCGCCA GGACACGTCG GAGCCCAGCT 1381 CCATGTGTTG TGATGTCGAG CACACACCCC AGATGATCGA ATCAAACCAA AGTCCAAGAT 1441 ATACACCATT TTCTGTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT TTCCCTTTCT 1501 CTCGGTCTCG ATTCTGCGTA GTAATGAACC TTGCCAAAGT GGTTGGTATT TCGATTTCTT 1561 GGCCTCTCCT CTCTTGTGGA AAGGCGTAGG AATGAGCTAC CACTACCCGG TGGCGCGTTA 1621 AGTCGACAAT CAACCTCTGG ATTACAAAAT TTGTGAAAGA TTGACTGGTA TTCTTAACTA 1681 TGTTGCTCCT TTTACGCTAT GTGGATACGC TGCTTTAATG CCTTTGTATC ATGTTATTGT 1741 TTAC