Construct: ORF ccsbBroad304_00049
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012745.1_s304c1, BRDN0000410309
- Derived from:
- ccsbBroadEn_00049
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ALDH2 (217)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_00049
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 217 | ALDH2 | aldehyde dehydrogenase 2 fa... | NM_000690.4 | 100% | 100% | |
| 2 | human | 217 | ALDH2 | aldehyde dehydrogenase 2 fa... | NM_001204889.2 | 90.9% | 90.9% | 218_219ins141 |
| 3 | mouse | 11669 | Aldh2 | aldehyde dehydrogenase 2, m... | NM_009656.4 | 86.2% | 94.8% | (many diffs) |
| 4 | mouse | 11669 | Aldh2 | aldehyde dehydrogenase 2, m... | NM_001308450.1 | 78.2% | 85.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1617
- ORF length:
- 1551
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgtt gcgcgctgcc gcccgcttcg ggccccgcct gggccgccgc ctcttgtcag 121 ccgccgccac ccaggccgtg cctgccccca accagcagcc cgaggtcttc tgcaaccaga 181 ttttcataaa caatgaatgg cacgatgccg tcagcaggaa aacattcccc accgtcaatc 241 cgtccactgg agaggtcatc tgtcaggtag ctgaagggga caaggaagat gtggacaagg 301 cagtgaaggc cgcccgggcc gccttccagc tgggctcacc ttggcgccgc atggacgcat 361 cacacagggg ccggctgctg aaccgcctgg ccgatctgat cgagcgggac cggacctacc 421 tggcggcctt ggagaccctg gacaatggca agccctatgt catctcctac ctggtggatt 481 tggacatggt cctcaaatgt ctccggtatt atgccggctg ggctgataag taccacggga 541 aaaccatccc cattgacgga gacttcttca gctacacacg ccatgaacct gtgggggtgt 601 gcgggcagat cattccgtgg aatttcccgc tcctgatgca agcatggaag ctgggcccag 661 ccttggcaac tggaaacgtg gttgtgatga aggtagctga gcagacaccc ctcaccgccc 721 tctatgtggc caacctgatc aaggaggctg gctttccccc tggtgtggtc aacattgtgc 781 ctggatttgg ccccacggct ggggccgcca ttgcctccca tgaggatgtg gacaaagtgg 841 cattcacagg ctccactgag attggccgcg taatccaggt tgctgctggg agcagcaacc 901 tcaagagagt gaccttggag ctggggggga agagccccaa catcatcatg tcagatgccg 961 atatggattg ggccgtggaa caggcccact tcgccctgtt cttcaaccag ggccagtgct 1021 gctgtgccgg ctcccggacc ttcgtgcagg aggacatcta tgatgagttt gtggagcgga 1081 gcgttgcccg ggccaagtct cgggtggtcg ggaacccctt tgatagcaag accgagcagg 1141 ggccgcaggt ggatgaaact cagtttaaga agatcctcgg ctacatcaac acggggaagc 1201 aagagggggc gaagctgctg tgtggtgggg gcattgctgc tgaccgtggt tacttcatcc 1261 agcccactgt gtttggagat gtgcaggatg gcatgaccat cgccaaggag gagatcttcg 1321 ggccagtgat gcagatcctg aagttcaaga ccatagagga ggttgttggg agagccaaca 1381 attccacgta cgggctggcc gcagctgtct tcacaaagga tttggacaag gccaattacc 1441 tgtcccaggc cctccaggcg ggcactgtgt gggtcaactg ctatgatgtg tttggagccc 1501 agtcaccctt tggtggctac aagatgtcgg ggagtggccg ggagttgggc gagtacgggc 1561 tgcaggcata cactgaagtg aaaactgtca cagtcaaagt gcctcagaag aactcatacc 1621 caactttctt gtacaaagtg gttggtaagc ctatccctaa ccctctcctc ggtctcgatt 1681 ctacgtagta atgagctagc gctaaccggt ggcgcgttaa gtcgacaatc aacctctgga 1741 tta