Construct: ORF ccsbBroad304_00096
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009760.1_s304c1, BRDN0000406960
- Derived from:
- ccsbBroadEn_00096
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ARF5 (381)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_00096
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 381 | ARF5 | ADP ribosylation factor 5 | NM_001662.4 | 100% | 100% | |
| 2 | human | 378 | ARF4 | ADP ribosylation factor 4 | NM_001660.4 | 77% | 90% | (many diffs) |
| 3 | mouse | 11844 | Arf5 | ADP-ribosylation factor 5 | NM_007480.1 | 94.2% | 100% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 606
- ORF length:
- 540
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggg cctcaccgtg tccgcgctct tttcgcggat cttcgggaag aagcagatgc 121 ggattctcat ggttggcttg gatgcggctg gcaagaccac aatcctgtac aaactgaagt 181 tgggggagat tgtcaccacc atcccaacca taggcttcaa tgtagaaaca gtggaatata 241 agaacatctg tttcacagtc tgggacgtgg gaggccagga caagattcgg cctctgtggc 301 ggcactactt ccagaacact cagggcctca tctttgtggt ggacagtaat gaccgggagc 361 gggtccaaga atctgctgat gaactccaga agatgctgca ggaggacgag ctgcgggatg 421 cagtgctgct ggtatttgcc aacaagcagg acatgcccaa cgccatgccc gtgagcgagc 481 tgactgacaa gctggggcta cagcacttac gcagccgcac gtggtatgtc caggccacct 541 gtgccaccca aggcacaggt ctgtacgatg gtctggactg gctgtcccac gagctgtcaa 601 agcgctaccc aactttcttg tacaaagtgg ttggtaagcc tatccctaac cctctcctcg 661 gtctcgattc tacgtagtaa tgagctagcg ctaaccggtg gcgcgttaag tcgacaatca 721 acctctggat ta