Construct: ORF ccsbBroad304_00147
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002579.1_s304c1, BRDN0000406178
- Derived from:
- ccsbBroadEn_00147
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- BAG1 (573)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_00147
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 573 | BAG1 | BAG cochaperone 1 | NM_001349286.2 | 100% | 100% | |
| 2 | human | 573 | BAG1 | BAG cochaperone 1 | NM_001172415.2 | 83.9% | 83.9% | 0_1ins132 |
| 3 | human | 573 | BAG1 | BAG cochaperone 1 | NM_004323.6 | 79.4% | 79.4% | 1_213del |
| 4 | human | 573 | BAG1 | BAG cochaperone 1 | NM_001349299.2 | 75.5% | 75.5% | 0_1ins201 |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 888
- ORF length:
- 822
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgaa gaagaaaacc cggcgccgct cgacccggag cgaggagttg acccggagcg 121 aggagttgac cctgagtgag gaagcgacct ggagtgaaga ggcgacccag agtgaggagg 181 cgacccaggg cgaagagatg aatcggagcc aggaggtgac ccgggacgag gagtcgaccc 241 ggagcgagga ggtgaccagg gaggaaatgg cggcagctgg gctcaccgtg actgtcaccc 301 acagcaatga gaagcacgac cttcatgtta cctcccagca gggcagcagt gaaccagttg 361 tccaagacct ggcccaggtt gttgaagagg tcataggggt tccacagtct tttcagaaac 421 tcatatttaa gggaaaatct ctgaaggaaa tggaaacacc gttgtcagca cttggaatac 481 aagatggttg ccgggtcatg ttaattggga aaaagaacag tccacaggaa gaggttgaac 541 taaagaagtt gaaacatttg gagaagtctg tggagaagat agctgaccag ctggaagagt 601 tgaataaaga gcttactgga atccagcagg gttttctgcc caaggatttg caagctgaag 661 ctctctgcaa acttgatagg agagtaaaag ccacaataga gcagtttatg aagatcttgg 721 aggagattga cacactgatc ctgccagaaa atttcaaaga cagtagattg aaaaggaaag 781 gcttggtaaa aaaggttcag gcattcctag ccgagtgtga cacagtggag cagaacatct 841 gccaggagac tgagcggctg cagtctacaa actttgccct ggccgagtgc ccaactttct 901 tgtacaaagt ggttggtaag cctatcccta accctctcct cggtctcgat tctacgtagt 961 aatgagctag cgctaaccgg tggcgcgtta agtcgacaat caacctctgg atta