Construct: ORF ccsbBroad304_00217
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017295.1_s304c1, BRDN0000407589
- Derived from:
- ccsbBroadEn_00217
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CAPN6 (827)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_00217
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 827 | CAPN6 | calpain 6 | NM_014289.4 | 100% | 100% | |
| 2 | mouse | 12338 | Capn6 | calpain 6 | NM_007603.3 | 90.1% | 95.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1989
- ORF length:
- 1923
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggg tcctcctctg aagctcttca aaaaccagaa ataccaggaa ctgaagcagg 121 aatgcatcaa agacagcaga cttttctgtg atccaacatt tctgcctgag aatgattctc 181 ttttctacaa ccgactgctt cctggaaagg tggtgtggaa acgtccccag gacatctgtg 241 atgaccccca tctgattgtg ggcaacatta gcaaccacca gctgacccaa gggagactgg 301 ggcacaagcc aatggtttct gcattttcct gtttggctgt tcaggagtct cattggacaa 361 agacaattcc caaccataag gaacaggaat gggaccctca aaaaacagaa aaatacgctg 421 ggatatttca ctttcgtttc tggcattttg gagaatggac tgaagtggtg attgatgact 481 tgttgcccac cattaacgga gatctggtct tctctttctc cacttccatg aatgagtttt 541 ggaatgctct gctggaaaaa gcttatgcaa agctgctagg ctgttatgag gccctggatg 601 gtttgaccat cactgatatt attgtggact tcacgggcac attggctgaa actgttgaca 661 tgcagaaagg aagatacact gagcttgttg aggagaagta caagctattc ggagaactgt 721 acaaaacatt taccaaaggt ggtctgatct gctgttccat tgagtctccc aatcaggagg 781 agcaagaagt tgaaactgat tggggtctgc tgaagggcca tacctatacc atgactgata 841 ttcgcaaaat tcgtcttgga gagagacttg tggaagtctt cagtgctgag aaggtgtata 901 tggttcgcct gagaaacccc ttgggaagac aggaatggag tggcccctgg agtgaaattt 961 ctgaagagtg gcagcaactg actgcatcag atcgcaagaa cctggggctt gttatgtctg 1021 atgatggaga gttttggatg agcttggagg acttttgccg caactttcac aaactgaatg 1081 tctgccgcaa tgtgaacaac cctatttttg gccgaaagga gctggaatcg gtgttgggat 1141 gctggactgt ggatgatgat cccctgatga accgctcagg aggctgctat aacaaccgtg 1201 ataccttcct gcagaatccc cagtacatct tcactgtgcc tgaggatggg cacaaggtca 1261 ttatgtcact gcagcagaag gacctgcgca cttaccgccg aatgggaaga cctgacaatt 1321 acatcattgg ctttgagctc ttcaaggtgg agatgaaccg caaattccgc ctccaccacc 1381 tctacatcca ggagcgtgct gggacttcca cctatattga cacccgcaca gtgtttctga 1441 gcaagtacct gaagaagggc aactatgtgc ttgtcccaac catgttccag catggtcgca 1501 ccagcgagtt tctcctgaga atcttctctg aagtgcctgt ccagctcagg gaactgactc 1561 tggacatgcc caaaatgtcc tgctggaacc tggctcgtgg ctacccgaaa gtagttactc 1621 agatcactgt tcacagtgct gaggacctgg agaagaagta tgccaatgaa actgtaaacc 1681 catatttggt catcaaatgt ggaaaggagg aagtccgttc tcctgtccag aagaatacag 1741 ttcatgccat ttttgacacc caggccattt tctacagaag gaccactgac attcctatta 1801 tagtacaggt ctggaacagc cgaaaattct gtgatcagtt cttggggcag gttactctgg 1861 atgctgaccc cagcgactgc cgtgatctga agtctctgta cctgcgtaag aagggtggtc 1921 caactgccaa agtcaagcaa ggccacatca gcttcaaggt tatttccagc gatgatctca 1981 ctgagctcta cccaactttc ttgtacaaag tggttggtaa gcctatccct aaccctctcc 2041 tcggtctcga ttctacgtag taatgagcta gcgctaaccg gtggcgcgtt aagtcgacaa 2101 tcaacctctg gatta