Construct: ORF ccsbBroad304_00217

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF017295.1_s304c1, BRDN0000407589
Derived from:
ccsbBroadEn_00217
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
CAPN6 (827)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_00217
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 827 CAPN6 calpain 6 NM_014289.4 100% 100%
2 mouse 12338 Capn6 calpain 6 NM_007603.3 90.1% 95.1% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1989
ORF length:
1923
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatggg tcctcctctg aagctcttca aaaaccagaa ataccaggaa ctgaagcagg
121aatgcatcaa agacagcaga cttttctgtg atccaacatt tctgcctgag aatgattctc
181ttttctacaa ccgactgctt cctggaaagg tggtgtggaa acgtccccag gacatctgtg
241atgaccccca tctgattgtg ggcaacatta gcaaccacca gctgacccaa gggagactgg
301ggcacaagcc aatggtttct gcattttcct gtttggctgt tcaggagtct cattggacaa
361agacaattcc caaccataag gaacaggaat gggaccctca aaaaacagaa aaatacgctg
421ggatatttca ctttcgtttc tggcattttg gagaatggac tgaagtggtg attgatgact
481tgttgcccac cattaacgga gatctggtct tctctttctc cacttccatg aatgagtttt
541ggaatgctct gctggaaaaa gcttatgcaa agctgctagg ctgttatgag gccctggatg
601gtttgaccat cactgatatt attgtggact tcacgggcac attggctgaa actgttgaca
661tgcagaaagg aagatacact gagcttgttg aggagaagta caagctattc ggagaactgt
721acaaaacatt taccaaaggt ggtctgatct gctgttccat tgagtctccc aatcaggagg
781agcaagaagt tgaaactgat tggggtctgc tgaagggcca tacctatacc atgactgata
841ttcgcaaaat tcgtcttgga gagagacttg tggaagtctt cagtgctgag aaggtgtata
901tggttcgcct gagaaacccc ttgggaagac aggaatggag tggcccctgg agtgaaattt
961ctgaagagtg gcagcaactg actgcatcag atcgcaagaa cctggggctt gttatgtctg
1021atgatggaga gttttggatg agcttggagg acttttgccg caactttcac aaactgaatg
1081tctgccgcaa tgtgaacaac cctatttttg gccgaaagga gctggaatcg gtgttgggat
1141gctggactgt ggatgatgat cccctgatga accgctcagg aggctgctat aacaaccgtg
1201ataccttcct gcagaatccc cagtacatct tcactgtgcc tgaggatggg cacaaggtca
1261ttatgtcact gcagcagaag gacctgcgca cttaccgccg aatgggaaga cctgacaatt
1321acatcattgg ctttgagctc ttcaaggtgg agatgaaccg caaattccgc ctccaccacc
1381tctacatcca ggagcgtgct gggacttcca cctatattga cacccgcaca gtgtttctga
1441gcaagtacct gaagaagggc aactatgtgc ttgtcccaac catgttccag catggtcgca
1501ccagcgagtt tctcctgaga atcttctctg aagtgcctgt ccagctcagg gaactgactc
1561tggacatgcc caaaatgtcc tgctggaacc tggctcgtgg ctacccgaaa gtagttactc
1621agatcactgt tcacagtgct gaggacctgg agaagaagta tgccaatgaa actgtaaacc
1681catatttggt catcaaatgt ggaaaggagg aagtccgttc tcctgtccag aagaatacag
1741ttcatgccat ttttgacacc caggccattt tctacagaag gaccactgac attcctatta
1801tagtacaggt ctggaacagc cgaaaattct gtgatcagtt cttggggcag gttactctgg
1861atgctgaccc cagcgactgc cgtgatctga agtctctgta cctgcgtaag aagggtggtc
1921caactgccaa agtcaagcaa ggccacatca gcttcaaggt tatttccagc gatgatctca
1981ctgagctcta cccaactttc ttgtacaaag tggttggtaa gcctatccct aaccctctcc
2041tcggtctcga ttctacgtag taatgagcta gcgctaaccg gtggcgcgtt aagtcgacaa
2101tcaacctctg gatta

Download FASTA (ORF) (Full)