Construct: ORF ccsbBroad304_00223
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013609.1_s304c1, BRDN0000403504
- Derived from:
- ccsbBroadEn_00223
- DNA Barcode:
- None
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- CASP9 (842)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_00223
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 842 | CASP9 | caspase 9 | NM_001278054.1 | 57.9% | 49.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 555
- ORF length:
- 489
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgga cgaagcggat cggcggctcc tgcggcggtg ccggctgcgg ctggtggaag 121 agctgcaggt ggaccagctc tgggacgccc tgctgagccg cgagctgttc aggccccata 181 tgatcgagga catccagcgg gcaggctctg gatctcggcg ggatcaggcc aggcagctga 241 tcatagatct ggagactcga gggagtcagg ctcttccttt gttcatctcc tgcttagagg 301 acacaggcca ggacatgctg gcttcgtttc tgcgaactaa caggcaagca gcaaagttgt 361 cgaagccaac cctagaaaac cttaccccag tggtgctcag accagagatt cgcaaaccag 421 aggttctcag accggaaaca cccagaccag tggacattgg ttctggagga tttggAGAAG 481 CGGGTATAAG AGTCATATTT GAGGCGAATT TGGCACGTCC CATACGTTCC CAAAAAAACT 541 TCCCACATTT TTTTTGATAA GTTCTTGGGC GGGGGTGCAC TCCGTCTCTG AGTTGGAAAA 601 AATAATCATT CTCACACAGG GGGGGAGAGA GTGGGGGGAC TTGGTCGTGT TTTAGGGGGA 661 GTTGGGGGCG AAATAAGGGG CTGATATATA AGCTCCACAC AATATAAGGA CACTTACAAG 721 TGTGTGGACA CACCACAAAA AAAACAGAGT TAAAAAAATG CTTTCTTTGG GAAGTGTGTG 781 AAGCTAGCCC TTTATGTGTA GCCAATAAGA GCCGCAATAA ACAGTTTCCT CTCACTCTGT 841 TATATTCATG TTTTTGCAAG TTATAAATCG TGTATATTAA GATCACATGA TCTCCTATGC 901 GTCCTGCTAG AGATTTTCCA CTCTGAATAA AAGTGTCGGG GGGATCTCCC GTCACCAGAG 961 TCCCACAACA GACGGGCACA CTCTACTTTA AGCACTCAAC GCAAGCTTTA GTGAGGTTTA 1021 AGCAGTGGGT TCCCTAGTTA GCCAGAGAGC TCCCAGTCTC AGCTCGGGTC TAGCCAGAGA 1081 GCCCCGTTTT TGTATCGAGC TAGTCACTCA AATACAATAT TTCCGAAACG CGGCAATTCA 1141 GTGGTTCGTC CAATCCATGT CAGACCCGTA AGTCGCCTTC CTAATAAGGC ACGATCGTAC 1201 CACCTTACTT CCGCCAATCG GCATGCACGC GCTTTTTCTC TCCTTGTAGG GCAAAGTGCT 1261 AACTCATCGT TACCATGACA AAAGACGTCA AGAATATGGA TAAGTCTACA TTAAGCTTGC 1321 AGCTCCAGCT TATGTGCCCT TTAGTCAGGA TTGCTGCTGG CTTGGCGTTT TCCCC