Construct: ORF ccsbBroad304_00250
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001082.1_s304c1, BRDN0000401147
- Derived from:
- ccsbBroadEn_00250
- DNA Barcode:
- None
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- CD19 (930)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_00250
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 930 | CD19 | CD19 molecule | NM_001770.6 | 100% | 100% | |
| 2 | human | 930 | CD19 | CD19 molecule | NM_001178098.2 | 99.8% | 99.8% | 1486_1488delGCA |
| 3 | human | 930 | CD19 | CD19 molecule | XM_017023893.1 | 83.9% | 83.9% | 87_88ins267 |
| 4 | human | 930 | CD19 | CD19 molecule | XM_006721103.3 | 83.8% | 83.8% | 87_88ins267;1219_1221delGCA |
| 5 | human | 930 | CD19 | CD19 molecule | XR_950871.2 | 65.6% | 1_45del;988_989ins49;1169_1170ins495 | |
| 6 | human | 930 | CD19 | CD19 molecule | XM_011545981.2 | 50.2% | 50% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1734
- ORF length:
- 1668
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgcc acctcctcgc ctcctcttct tcctcctctt cctcaccccc atggaagtca 121 ggcccgagga acctctagtg gtgaaggtgg aagagggaga taacgctgtg ctgcagtgcc 181 tcaaggggac ctcagatggc cccactcagc agctgacctg gtctcgggag tccccgctta 241 aacccttctt aaaactcagc ctggggctgc caggcctggg aatccacatg aggcccctgg 301 ccatctggct tttcatcttc aacgtctctc aacagatggg gggcttctac ctgtgccagc 361 cggggccccc ctctgagaag gcctggcagc ctggctggac agtcaatgtg gagggcagcg 421 gggagctgtt ccggtggaat gtttcggacc taggtggcct gggctgtggc ctgaagaaca 481 ggtcctcaga gggccccagc tccccttccg ggaagctcat gagccccaag ctgtatgtgt 541 gggccaaaga ccgccctgag atctgggagg gagagcctcc gtgtctccca ccgagggaca 601 gcctgaacca gagcctcagc caggacctca ccatggcccc tggctccaca ctctggctgt 661 cctgtggggt accccctgac tctgtgtcca ggggccccct ctcctggacc catgtgcacc 721 ccaaggggcc taagtcattg ctgagcctag agctgaagga cgatcgcccg gccagagata 781 tgtgggtaat ggagacgggt ctgttgttgc cccgggccac agctcaagac gctggaaagt 841 attattgtca ccgtggcaac ctgaccatgt cattccacct ggagatcact gctcggccag 901 tactatggca ctggctgctg aggactggtg gctggaaggt ctcagctgtg actttggctt 961 atctgatctt ctgcctgtgt tcccttgtgg gcattcttca tcttcaaaga gccctggtcc 1021 tgaggaggaa aagaaagcga atgactgacc ccaccaggag attcttcaaa gtgacgcctc 1081 ccccaggaag cgggccccag aaccagtacg ggaacgtgct gtctctcccc acacccacct 1141 caggcctcgg acgcgcccag cgttgggccg caggcctggg gggcactgcc ccgtcttatg 1201 gaaacccgag cagcgacgtc caggcggatg gagccttggg gtcccggagc ccgccgggag 1261 tgggcccaga agaagaggaa ggggagggct atgaggaacc tgacagtgag gaggactccg 1321 agttctatga gaacgactcc aaccttgggc aggaccagct ctcccaggat ggcagcggct 1381 acgagaaccc tgaggatgag cccctgggtc ctgaggatga agactccttc tccaacgctg 1441 agtcttatga gaacgaggat gaagagctga cccagccggt cgccaggaca atggacttcc 1501 tgagccctca tgggtcagcc tgggacccca gccgggaagc aacctccctg gggtcccagt 1561 cctatgagga tatgagagga atcctgtatg cagcccccca gctccgctcc attcggggcc 1621 agcctggacc caatcatgag gaagatgcag actcttatga gaacatggat aatcccgatg 1681 ggccagaccc agcctgggga ggagggggcc gcatgggcac ctggagcacc aggtgaccaa 1741 ctttcttgta caaagtggtt ggtaagccta tccctaaccc tctcctcggt ctcgattcta 1801 cgtagtaatg agctagcgct aaccggtggc gcgttaagtc gacaatcaac ctctggatta