Construct: ORF ccsbBroad304_00318

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF001896.1_s304c1, BRDN0000399016
Derived from:
ccsbBroadEn_00318
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
AP2M1 (1173)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_00318
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 1173 AP2M1 adaptor related protein com... NM_004068.4 100% 100%
2 human 1173 AP2M1 adaptor related protein com... NM_001025205.1 99.5% 99.5% 419_420insTCAGCA
3 human 1173 AP2M1 adaptor related protein com... NM_001311198.1 94.5% 94.5% 74_148del
4 mouse 11773 Ap2m1 adaptor-related protein com... NM_009679.3 92.6% 100% (many diffs)
5 mouse 11773 Ap2m1 adaptor-related protein com... NM_001302970.1 92.1% 99.5% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1371
ORF length:
1305
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgat tggaggctta ttcatctata atcacaaggg ggaggtgctc atctcccgag
121tctaccgaga tgacatcggg aggaacgcag tggatgcctt tcgggtcaat gttatccatg
181cccggcagca ggtgcgcagc cccgtcacca acattgctcg caccagcttc ttccacgtta
241agcggtccaa catttggctg gcagcagtca ccaagcagaa tgtcaacgct gccatggtct
301tcgaattcct ctataagatg tgtgacgtga tggctgccta ctttggcaag atcagcgagg
361aaaacatcaa gaacaatttt gtgctcatat atgagctgct ggatgagatt ctagactttg
421gctacccaca gaattccgag acaggcgcgc tgaaaacctt catcacgcag cagggcatca
481agagtcagca tcagacaaaa gaagagcagt cacagatcac cagccaggta actgggcaga
541ttggctggcg gcgagagggt atcaagtatc gtcggaatga gctcttcctg gatgtgctgg
601agagtgtgaa cctgctcatg tccccacaag ggcaggtgct gagtgcccat gtgtcgggcc
661gggtggtgat gaagagctac ctgagtggca tgcctgaatg caagtttggg atgaatgaca
721agattgttat tgaaaagcag ggcaaaggca cagctgatga aacaagcaag agcgggaagc
781aatcaattgc cattgatgac tgcaccttcc accagtgtgt gcgactcagc aagtttgact
841ctgaacgcag catcagcttt atcccgccag atggagagtt tgagcttatg aggtatcgca
901caaccaagga catcatcctt cccttccggg tgatcccgct agtgcgagaa gtgggacgca
961ccaaactgga ggtcaaggtg gtcatcaagt ccaactttaa accctcactg ctggctcaga
1021agatcgaggt gaggatccca accccactga acacaagcgg ggtgcaggtg atctgcatga
1081aggggaaggc caagtacaag gccagcgaga atgccatcgt gtggaagatc aagcgcatgg
1141caggcatgaa ggaatcgcag atcagcgcag agattgagct tctgcctacc aacgacaaga
1201agaaatgggc tcgacccccc atttccatga actttgaggt gccattcgcg ccctctggcc
1261tcaaggtgcg ctacttgaag gtgtttgaac cgaagctgaa ctacagcgac catgatgtca
1321tcaaatgggt gcgctacatt ggccgcagtg gcatttatga aactcgctgc tacccaactt
1381tcttgtacaa agtggttggt aagcctatcc ctaaccctct cctcggtctc gattctacgt
1441agtaatgagc tagcgctaac cggtggcgcg ttaagtcgac aatcaacctc tggatta

Download FASTA (ORF) (Full)