Transcript: Mouse NM_001302970.1

Mus musculus adaptor-related protein complex 2, mu 1 subunit (Ap2m1), transcript variant 2, mRNA.

Source:
NCBI, updated 2017-06-04
Taxon:
Mus musculus (mouse)
Gene:
Ap2m1 (11773)
Length:
2931
CDS:
166..1467

Additional Resources:

NCBI RefSeq record:
NM_001302970.1
NBCI Gene record:
Ap2m1 (11773)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_001302970.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000111747 CCGTACTACCAAGGACATCAT pLKO.1 990 CDS 100% 4.950 6.930 N Ap2m1 n/a
2 TRCN0000294658 GCGACCATGATGTCATCAAAT pLKO_005 1400 CDS 100% 13.200 10.560 N Ap2m1 n/a
3 TRCN0000381860 GCGACCATGATGTCATCAAAT pLKO_005 1400 CDS 100% 13.200 10.560 N AP2M1 n/a
4 TRCN0000294718 ATTGGCCGCAGTGGCATTTAT pLKO_005 1432 CDS 100% 15.000 10.500 N Ap2m1 n/a
5 TRCN0000294673 TCCAGTTACAAACCCAATAAA pLKO_005 1897 3UTR 100% 15.000 10.500 N Ap2m1 n/a
6 TRCN0000379827 ACTCTGCTTTGGGATAGTATG pLKO_005 1848 3UTR 100% 10.800 7.560 N Ap2m1 n/a
7 TRCN0000381884 CAAAGGCACAGCTGATGAAAC pLKO_005 837 CDS 100% 10.800 7.560 N Ap2m1 n/a
8 TRCN0000111745 CCACTCTGCTTTGGGATAGTA pLKO.1 1846 3UTR 100% 5.625 3.938 N Ap2m1 n/a
9 TRCN0000111746 CCCACCAACGATAAGAAGAAA pLKO.1 1279 CDS 100% 5.625 3.938 N Ap2m1 n/a
10 TRCN0000111748 GCCTGAGTGCAAGTTTGGAAT pLKO.1 786 CDS 100% 4.950 3.465 N Ap2m1 n/a
11 TRCN0000287805 GCCTGAGTGCAAGTTTGGAAT pLKO_005 786 CDS 100% 4.950 3.465 N Ap2m1 n/a
12 TRCN0000379540 TGCAGTGGGAAGGTTGACTAC pLKO_005 1771 3UTR 100% 4.050 2.835 N Ap2m1 n/a
13 TRCN0000060240 CCATGAACTTTGAGGTGCCAT pLKO.1 1319 CDS 100% 2.640 1.848 N AP2M1 n/a
14 TRCN0000111749 CGACGGAGAGTTTGAACTCAT pLKO.1 963 CDS 100% 4.950 2.475 Y Ap2m1 n/a
15 TRCN0000060238 GTGGTCATCAAGTCCAACTTT pLKO.1 1072 CDS 100% 5.625 3.375 N AP2M1 n/a
16 TRCN0000333063 GTGGTCATCAAGTCCAACTTT pLKO_005 1072 CDS 100% 5.625 3.375 N AP2M1 n/a
17 TRCN0000182716 CCTGAGTTCAATTCCCAGCAA pLKO.1 2810 3UTR 100% 2.640 1.320 Y BC028528 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001302970.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_06008 pDONR223 100% 92.5% 99.7% None (many diffs) n/a
2 ccsbBroad304_06008 pLX_304 0% 92.5% 99.7% V5 (many diffs) n/a
3 TRCN0000470339 TCACACTTGAAGGGCTCTATCACC pLX_317 35.1% 92.5% 99.7% V5 (many diffs) n/a
4 ccsbBroadEn_00318 pDONR223 100% 92.1% 99.5% None (many diffs) n/a
5 ccsbBroad304_00318 pLX_304 0% 92.1% 99.5% V5 (many diffs) n/a
6 TRCN0000472776 GCTCACACTCGTGAGCATTCACCT pLX_317 43.5% 92.1% 99.5% V5 (many diffs) n/a
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