Construct: ORF ccsbBroad304_00353
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009232.1_s304c1, BRDN0000410448
- Derived from:
- ccsbBroadEn_00353
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- COX6B1 (1340)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_00353
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1340 | COX6B1 | cytochrome c oxidase subuni... | NM_001863.5 | 100% | 100% | |
| 2 | mouse | 110323 | Cox6b1 | cytochrome c oxidase, subun... | NM_025628.2 | 85.8% | 84.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 324
- ORF length:
- 258
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggc ggaagacatg gagaccaaaa tcaagaacta caagaccgcc ccttttgaca 121 gccgcttccc caaccagaac cagactagaa actgctggca gaactacctg gacttccacc 181 gctgtcagaa ggcaatgacc gctaaaggag gcgatatctc tgtgtgcgaa tggtaccagc 241 gtgtgtacca gtccctctgc cccacatcct gggtcacaga ctgggatgag caacgggctg 301 aaggcacgtt tcccgggaag atctgcccaa ctttcttgta caaagtggtt ggtaagccta 361 tccctaaccc tctcctcggt ctcgattcta cgtagtaatg agctagcgct aaccggtggc 421 gcgttaagtc gacaatcaac ctctggatta