Construct: ORF ccsbBroad304_00356
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005713.1_s304c1, BRDN0000462321
- Derived from:
- ccsbBroadEn_00356
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CPD (1362)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_00356
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1362 | CPD | carboxypeptidase D | NM_001304.5 | 100% | 100% | |
| 2 | human | 1362 | CPD | carboxypeptidase D | NM_001199775.1 | 82% | 81.9% | 0_1ins741;2_3delTGinsAC;5G>A |
| 3 | mouse | 12874 | Cpd | carboxypeptidase D | NM_007754.2 | 87.6% | 90.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 4206
- ORF length:
- 4140
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggc gagcggccgg gacgagcggc cgccttggcg gctagggcgg ctcctgttgc 121 tcatgtgcct gctgctgctg gggagctcgg cccgggcggc tcacatcaag aaggcggagg 181 cgactaccac aactacgagc gcgggcgccg aggcggccga gggccagttc gaccgctact 241 accacgaaga ggagttggag tcggcgctga gggaggcggc ggccgcgggc ctccccggcc 301 tggcccgcct ctttagcatc ggccgctcgg tggaaggccg gccgctgtgg gtgcttcgcc 361 tcaccgccgg cctggggtcg ctaatccctg agggcgacgc ggggcctgac gctgccgggc 421 ccgacgctgc ggggccgctg ctgcccggcc ggccccaggt gaagctggtg ggcaacatgc 481 atggcgacga gaccgtgtcg cgccaggtgt tgatctactt ggcccgcgag ctggcggccg 541 gctaccgccg cggggacccg cgcctggtcc gcctgctcaa caccaccgac gtgtacctgc 601 tgcccagcct caaccccgat ggcttcgagc gtgcccgcga gggcgactgt ggcttcggcg 661 acggcggccc gtccggggcc agcggccgcg acaatagtcg cggccgcgac ctcaaccgaa 721 gctttcccga ccagtttagc accggcgaac cccccgccct ggacgaggtg cccgaggtgc 781 gcgccctcat cgagtggatc cgcaggaaca agtttgtgct ttctggaaat ctgcatggtg 841 gctcagtggt agcaagctat ccttttgatg attctccaga acataaggcc actggaatct 901 atagcaaaac ctcagatgat gaagtattta aatacttggc aaaagcttat gcttcaaacc 961 accccataat gaaaactggt gagcctcatt gtccaggaga tgaagacgag actttcaaag 1021 atggaatcac aaacggcgca cattggtatg atgtggaagg tggtatgcaa gattacaatt 1081 atgtgtgggc caactgtttt gagatcacat tagaactgtc ttgttgcaag tacccacctg 1141 cttcacagct tcgacaggaa tgggagaaca atcgtgagtc tttgatcaca ttgattgaaa 1201 aggttcacat tggagtgaaa ggatttgtta aagattccat aacaggatct gggttagaga 1261 atgcaaccat ctcagtggct ggtattaatc ataatatcac aacaggcaga tttggtgatt 1321 tctaccgatt acttgttcct ggaacttaca accttacagt agttttaact gggtatatgc 1381 cattgactgt tactaatgta gtggtgaaag aaggaccagc cacagaggtg gatttttctc 1441 ttaggccaac tgtaacttca gtaatccctg acacgacaga ggctgtatca actgctagca 1501 cagttgctat acctaatatt ctttctggaa catcatcctc ctaccagcca attcagccaa 1561 aggactttca ccaccaccat ttccctgata tggaaatctt cttgagaagg tttgccaatg 1621 aatatcctaa cattacccgg ctttattcct tgggaaaatc agtagagtca agagaacttt 1681 atgtgatgga gatatctgat aatccgggtg tccatgaacc aggtgaacca gaatttaagt 1741 acattggaaa tatgcatgga aatgaagtgg ttggaagaga actgctgttg aacctcatag 1801 aatacctttg taagaacttt ggaacagacc ctgaagtcac agatttggtt cataacacta 1861 gaattcacct tatgccatcc atgaatcctg atgggtatga aaagtcccag gaaggagatt 1921 caataagtgt aattggcaga aacaacagca acaactttga cctgaaccga aatttcccag 1981 accagtttgt tcagatcaca gatcctacgc aaccagaaac tattgctgta atgagctgga 2041 tgaagtccta tccatttgta ctttcagcaa acctgcatgg aggttctttg gtggttaact 2101 acccttttga tgatgatgaa caaggacttg ccacatatag taaatcacca gatgatgctg 2161 tgttccaaca aatagcactt tcttattcca aggaaaattc ccagatgttt caaggtagac 2221 cttgcaagaa tatgtatcct aatgaatatt ttcctcatgg aataacaaat ggagctagtt 2281 ggtataatgt gccaggagga atgcaggact ggaactattt acaaacaaat tgctttgaag 2341 tgactattga actaggttgt gtgaaatatc cacttgagaa agagctgcca aacttttggg 2401 aacagaatcg aagatcacta atccagttta tgaaacaggt tcatcagggc gtcagaggat 2461 ttgttctaga tgccacagat ggcaggggta tattaaatgc caccattagt gttgctgaga 2521 ttaatcaccc agtgactact tacaaaactg gagattactg gcgtctcttg gttccaggaa 2581 cttataaaat cacagcatct gctcgagggt ataatccagt taccaagaat gtgactgtca 2641 agagtgaagg cgctattcag gtcaacttca cacttgttcg atcctcaaca gattcaaaca 2701 atgaatcaaa gaaaggaaaa ggggctagca gcagcaccaa tgatgccagt gatccaacta 2761 ctaaagagtt tgaaacttta attaaagacc tttcagcgga gaatggtttg gaaagcctca 2821 tgttacgctc ctcctcaaat ctggctctgg ctctttatcg ataccattcc tacaaagact 2881 tatcagagtt tctgagagga cttgtaatga actatccaca tattacaaat cttaccaatt 2941 tgggacagag cactgaatat cgtcacattt ggtcccttga aatctccaat aagcccaatg 3001 tatctgagcc tgaagaacca aagattcgtt ttgttgctgg tatccatgga aatgcgccag 3061 ttggaactga actgcttttg gctctggcag aatttctctg cctgaactac aaaaagaacc 3121 cagctgttac ccaattggtt gacaggacta ggattgtgat tgtcccttct ctaaatccag 3181 atgggcgaga gagagctcaa gagaaagact gtacttcaaa aataggacaa acaaatgctc 3241 gtggcaaaga tttggataca gacttcacaa ataatgcctc ccaacctgag accaaagcca 3301 tcattgaaaa tttgattcaa aaacaggact ttagtctttc tgttgcctta gatggtggtt 3361 ccatgctggt cacatatcct tatgacaagc cagtacagac agtggaaaat aaagagactc 3421 tgaagcattt ggcatctctt tatgcaaata atcatccatc catgcacatg ggtcagccca 3481 gttgcccaaa taaatcagat gagaatattc caggaggagt aatgcgtgga gcagaatggc 3541 atagtcacct gggcagcatg aaggattata gtgtcaccta tggccattgt ccggaaatca 3601 cagtatacac aagctgctgt tactttccta gtgctgcacg actcccttcc ttgtgggcag 3661 acaataagag atctcttctt agtatgttag tggaggttca caagggagtt catggatttg 3721 ttaaagataa gactggaaag ccaatctcta aagcagtcat tgtacttaat gaaggaataa 3781 aggtacaaac aaaagaggga ggttatttcc atgtactctt agcgccaggt gtccataaca 3841 ttattgccat cgctgatggg taccagcaac aacattcaca ggtctttgtg catcatgatg 3901 cagctagttc tgtggtgata gtctttgaca cagataaccg gatatttggt ttgccaaggg 3961 agcttgtggt aactgtatca ggtgctacta tgtcggcatt gatcctaaca gcttgcatta 4021 tttggtgcat ctgctcaatc aagtctaata gacacaagga tggctttcat cggctcaggc 4081 agcatcatga tgagtatgaa gatgaaattc gcatgatgtc taccggctcc aagaagtccc 4141 tcctaagcca tgagttccag gatgaaacag acactgaaga ggaaacatta tattctagca 4201 aacattgccc aactttcttg tacaaagtgg ttggtaagcc tatccctaac cctctcctcg 4261 gtctcgattc tacgtagtaa tgagctagcg ctaaccggtg gcgcgttaag tcgacaatca 4321 acctctggat ta