Construct: ORF ccsbBroad304_00391

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF010559.1_s304c1, BRDN0000405565
Derived from:
ccsbBroadEn_00391
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
CTH (1491)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_00391
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 1491 CTH cystathionine gamma-lyase NM_001902.6 100% 100%
2 human 1491 CTH cystathionine gamma-lyase NM_001190463.1 92% 92% 250_251ins96
3 human 1491 CTH cystathionine gamma-lyase NM_153742.4 89.1% 89.1% 454_455ins132
4 human 1491 CTH cystathionine gamma-lyase XM_005270509.3 73% 73% 0_1ins327
5 human 1491 CTH cystathionine gamma-lyase XM_017000416.2 53% 53% 0_1ins570
6 mouse 107869 Cth cystathionase (cystathionin... NM_145953.2 80.2% 83.9% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1281
ORF length:
1215
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61tttgcatgca ggaaaaagac gcctcctcac aaggtttcct gccacacttc caacatttcg
121ccacgcaggc gatccatgtg ggccaggatc cagagcaatg gacctccagg gctgtagtgc
181cccccatctc actgtccacc acgttcaagc aaggggcgcc tggccagcac tcgggttttg
241aatatagccg ttctggaaat cccactagga attgccttga aaaagcagtg gcagcactgg
301atggggctaa gtactgtttg gcctttgctt caggtttagc agccactgta actattaccc
361atcttttaaa agcaggagac caaattattt gtatggatga tgtgtatgga ggtacaaaca
421ggtacttcag gcaagtggca tctgaatttg gattaaagat ttcttttgtt gattgttcca
481aaatcaaatt actagaggca gcaattacac cagaaaccaa gcttgtttgg atcgaaaccc
541ccacaaaccc cacccagaag gtgattgaca ttgaaggctg tgcacatatt gtccataagc
601atggagacat tattttggtc gtggataaca cttttatgtc accatatttc cagcgccctt
661tggctctggg agctgatatt tctatgtatt ctgcaacaaa atacatgaat ggccacagtg
721atgttgtaat gggcctggtg tctgttaatt gtgaaagcct tcataataga cttcgtttct
781tgcaaaactc tcttggagca gttccatctc ctattgattg ttacctctgc aatcgaggtc
841tgaagactct acatgtccga atggaaaagc atttcaaaaa cggaatggca gttgcccagt
901tcctggaatc taatccttgg gtagaaaagg ttatttatcc tgggctgccc tctcatccac
961agcatgagtt ggtgaagcgt cagtgtacag gttgtacagg gatggtcacc ttttatatta
1021agggcactct tcagcatgct gagattttcc tcaagaacct aaagctattt actctggccg
1081agagcttggg aggattcgaa agccttgctg agcttccggc aatcatgact catgcatcag
1141ttcttaagaa tgacagagat gtccttggaa ttagtgacac actgattcga ctttctgtgg
1201gcttagagga tgaggaagac ctactggaag atctagatca agctttgaag gcagcacacc
1261ctccaagtgg aagtcacagc tacccaactt tcttgtacaa agtggttggt aagcctatcc
1321ctaaccctct cctcggtctc gattctacgt agtaatgagc tagcgctaac cggtggcgcg
1381ttaagtcgac aatcaacctc tggatta

Download FASTA (ORF) (Full)