Transcript: Human XM_005270509.3

PREDICTED: Homo sapiens cystathionine gamma-lyase (CTH), transcript variant X1, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
CTH (1491)
Length:
4258
CDS:
546..1436

Additional Resources:

NCBI RefSeq record:
XM_005270509.3
NBCI Gene record:
CTH (1491)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_005270509.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000078263 GCACCTCATTATCTTTCATAA pLKO.1 1546 3UTR 100% 13.200 18.480 N CTH n/a
2 TRCN0000078264 CCTTCATAATAGACTTCGTTT pLKO.1 911 CDS 100% 4.950 6.930 N CTH n/a
3 TRCN0000078265 GCCTTCATAATAGACTTCGTT pLKO.1 910 CDS 100% 3.000 4.200 N CTH n/a
4 TRCN0000416390 TGGAAGTCACAGCTAGTATTC pLKO_005 1421 CDS 100% 10.800 8.640 N CTH n/a
5 TRCN0000416589 TCAAGAACCTAAAGCTATTTA pLKO_005 1204 CDS 100% 15.000 10.500 N CTH n/a
6 TRCN0000412672 ATCAAATCTTCCTGAGTAATT pLKO_005 1475 3UTR 100% 13.200 9.240 N CTH n/a
7 TRCN0000434478 ATTACAGGTCAATTCTGTTAA pLKO_005 1623 3UTR 100% 13.200 9.240 N CTH n/a
8 TRCN0000222704 GCCCAGTTCCTGGAATCTAAT pLKO.1 1047 CDS 100% 13.200 9.240 N CTH n/a
9 TRCN0000423468 GTGCTACTTTGGGAGATTATG pLKO_005 1695 3UTR 100% 13.200 9.240 N CTH n/a
10 TRCN0000078266 CCACTAGGAATTGCCTTGAAA pLKO.1 415 5UTR 100% 5.625 3.938 N CTH n/a
11 TRCN0000138140 GATTGAGACCATCCTGGCTAA pLKO.1 2046 3UTR 100% 4.050 2.025 Y LOC441087 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_005270509.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_00391 pDONR223 100% 73% 73% None 0_1ins327 n/a
2 ccsbBroad304_00391 pLX_304 0% 73% 73% V5 0_1ins327 n/a
3 TRCN0000474979 TTACAATAAATATTGATCCCTAGT pLX_317 36.6% 73% 73% V5 0_1ins327 n/a
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