Construct: ORF ccsbBroad304_00406
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007103.1_s304c1, BRDN0000400179
- Derived from:
- ccsbBroadEn_00406
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CYP1A1 (1543)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_00406
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1543 | CYP1A1 | cytochrome P450 family 1 su... | NM_000499.5 | 100% | 100% | |
| 2 | human | 1543 | CYP1A1 | cytochrome P450 family 1 su... | NM_001319217.2 | 100% | 100% | |
| 3 | human | 1543 | CYP1A1 | cytochrome P450 family 1 su... | NM_001319216.2 | 94.3% | 94.1% | 1166_1167ins87 |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1602
- ORF length:
- 1536
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgct tttcccaatc tccatgtcgg ccacggagtt tcttctggcc tctgtcatct 121 tctgtctggt attctgggta atcagggcct caagacctca ggtccccaaa ggcctgaaga 181 atccaccagg gccatggggc tggcctctga ttgggcacat gctgaccctg ggaaagaacc 241 cgcacctggc actgtcaagg atgagccagc agtatgggga cgtgctgcag atccgaattg 301 gctccacacc cgtggtggtg ctgagcggcc tggacaccat ccggcaggcc ctggtgcggc 361 agggcgatga tttcaagggc cggcccgacc tctacacctt caccctcatc agtaatggtc 421 agagcatgtc cttcagccca gactctggac cagtgtgggc tgcccgccgg cgcctggccc 481 agaatggcct gaaaagtttc tccattgcct ctgacccagc ctcctcaacc tcctgctacc 541 tggaagagca tgtgagcaag gaggctgagg tcctgataag cacgttgcag gagctgatgg 601 cagggcctgg gcactttaac ccctacaggt atgtggtggt atcagtgacc aatgtcatct 661 gtgccatttg ctttggccgg cgctatgacc acaaccacca agaactgctt agcctagtca 721 acctgaataa taatttcggg gaggtggttg gctctggaaa cccagctgac ttcatcccta 781 ttcttcgcta cctacccaac ccttccctga atgccttcaa ggacctgaat gagaagttct 841 acagcttcat gcagaagatg gtcaaggagc actacaaaac ctttgagaag ggccacatcc 901 gggacatcac agacagcctg attgagcact gtcaggagaa gcagctggat gagaacgcca 961 atgtccagct gtcagatgag aagatcatta acatcgtctt ggacctcttt ggagctgggt 1021 ttgacacagt cacaactgct atctcctgga gcctcatgta tttggtgatg aaccccaggg 1081 tacagagaaa gatccaagag gagctagaca cagtgattgg caggtcacgg cggccccggc 1141 tctctgacag atcccatctg ccctatatgg aggccttcat cctggagacc ttccgacact 1201 cttccttcgt ccccttcacc atcccccaca gcacaacaag agacacaagt ttgaaaggct 1261 tttacatccc caaggggcgt tgtgtctttg taaaccagtg gcagatcaac catgaccaga 1321 agctatgggt caacccatct gagttcctac ctgaacggtt tctcacccct gatggtgcta 1381 tcgacaaggt gttaagtgag aaggtgatta tctttggcat gggcaagcgg aagtgtatcg 1441 gtgagaccat tgcccgctgg gaggtctttc tcttcctggc tatcctgctg caacgggtgg 1501 aattcagcgt gccactgggc gtgaaggtgg acatgacccc catctatggg ctaaccatga 1561 agcatgcctg ctgtgagcac ttccaaatgc agctgcgctc ttacccaact tacttgtaca 1621 aagtggttgg taagcctatc cctaaccctc tcctcggtct cgattctacg tagtaatgag 1681 ctagcgctaa ccggtggcgc gttaagtcga caatcaacct ctggatta