Construct: ORF ccsbBroad304_00420
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005908.1_s304c1, BRDN0000403685
- Derived from:
- ccsbBroadEn_00420
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DAP (1611)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_00420
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1611 | DAP | death associated protein | NM_004394.3 | 100% | 100% | |
| 2 | human | 1611 | DAP | death associated protein | NM_001291963.2 | 40.1% | 27.5% | 151_152ins43;264_612del |
| 3 | mouse | 223453 | Dap | death-associated protein | NM_146057.3 | 86.6% | 96% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 372
- ORF length:
- 306
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgtc ttcgcctccc gaagggaaac tagagactaa agctggacac ccgcccgccg 121 tgaaagctgg tggaatgcga attgtgcaga aacacccaca tacaggagac accaaagaag 181 agaaagacaa ggatgaccag gaatgggaaa gccccagtcc acctaaaccc actgtgttca 241 tctctggggt catcgcccgg ggtgacaaag atttcccccc ggcggctgcg caggtggctc 301 accagaagcc gcatgcctcc atggacaagc atccttcccc aagaacccag cacatccagc 361 agccacgcaa gtgcccaact ttcttgtaca aagtggttgg taagcctatc cctaaccctc 421 tcctcggtct cgattctacg tagtaatgag ctagcgctaa ccggtggcgc gttaagtcga 481 caatcaacct ctggatta