Construct: ORF ccsbBroad304_00435

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF004838.1_s304c1, BRDN0000410531
Derived from:
ccsbBroadEn_00435
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
DEFA3 (1668)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_00435
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 1668 DEFA3 defensin alpha 3 NM_005217.4 100% 100%
2 human 728358 DEFA1B defensin alpha 1B NM_001042500.1 99.6% 98.9% 194C>A
3 human 1667 DEFA1 defensin alpha 1 NM_004084.3 99.6% 98.9% 194C>A
4 human 1668 DEFA3 defensin alpha 3 XM_011534741.2 93% 93% 1_21del
5 human 728358 DEFA1B defensin alpha 1B NM_001302265.1 92.7% 92% 1_21del;215C>A
6 human 1667 DEFA1 defensin alpha 1 XM_011534740.3 92.7% 92% 1_21del;215C>A
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
348
ORF length:
282
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgag gaccctcgcc atccttgctg ccattctcct ggtggccctg caggcccagg
121ctgagccact ccaggcaaga gctgatgagg ttgctgcagc cccggagcag attgcagcgg
181acatcccaga agtggttgtt tcccttgcat gggacgaaag cttggctcca aagcatccag
241gctcaaggaa aaacatggac tgctattgca gaataccagc gtgcattgca ggagaacgtc
301gctatggaac ctgcatctac cagggaagac tctgggcatt ctgctgctgc ccaactttct
361tgtacaaagt ggttggtaag cctatcccta accctctcct cggtctcgat tctacgtagt
421aatgagctag cgctaaccgg tggcgcgtta agtcgacaat caacctctgg atta

Download FASTA (ORF) (Full)