Construct: ORF ccsbBroad304_00461

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF013479.1_s304c1, BRDN0000403902
Derived from:
ccsbBroadEn_00461
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
DPYSL2 (1808)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_00461
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 1808 DPYSL2 dihydropyrimidinase like 2 NM_001386.6 99.9% 100% 426T>C
2 human 1808 DPYSL2 dihydropyrimidinase like 2 NM_001244604.2 93.6% 93.7% 0_1ins108;318T>C
3 human 1808 DPYSL2 dihydropyrimidinase like 2 NM_001197293.3 83.8% 83% (many diffs)
4 mouse 12934 Dpysl2 dihydropyrimidinase-like 2 NM_009955.3 91.4% 98.7% (many diffs)
5 mouse 12934 Dpysl2 dihydropyrimidinase-like 2 XM_006518508.3 76.9% 82.4% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1782
ORF length:
1716
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgtc ttatcagggg aagaaaaata ttccacgcat cacgagcgat cgtcttctga
121tcaaaggagg taaaattgtt aatgatgacc agtcgttcta tgcagacata tacatggaag
181atgggttgat caagcaaata ggagaaaatc tgattgtgcc aggaggagtg aagaccatcg
241aggcccactc ccggatggtg atccccggag gaattgacgt ccacactcgt ttccagatgc
301ctgatcaggg aatgacgtct gctgatgatt tcttccaagg aaccaaggcg gccctggctg
361ggggaaccac tatgatcatt gaccacgttg ttcctgagcc tgggacaagc ctgctcgctg
421cctttgacca gtggagggaa tgggccgaca gcaagtcctg ctgtgactac tctctgcatg
481tggacatcag cgagtggcat aagggcatcc aggaggagat ggaagcgctt gtgaaggatc
541acggggtaaa ttccttcctc gtgtacatgg ctttcaaaga tcgcttccag ctaacggatt
601gccagattta tgaagtactg agtgtgatcc gggatattgg cgccatagcc caagtccacg
661cagaaaatgg cgacatcatt gcagaggagc agcagaggat cctggatctg ggcatcacgg
721gccccgaggg acatgtgctg agccgacctg aggaggtcga ggccgaagcc gtgaatcgtg
781ccatcaccat cgccaaccag accaactgcc cgctgtatat caccaaggtg atgagcaaaa
841gctctgctga ggtcatcgcc caggcacgga agaagggaac tgtggtgtat ggcgagccca
901tcactgccag cttgggaacg gacggctccc attactggag caagaactgg gccaaggctg
961ctgcctttgt cacctcccca cccttgagcc ctgatccaac cactccagac tttctcaact
1021ccttgctgtc ctgtggagac ctccaggtca cgggcagtgc ccattgcacg tttaacactg
1081cccagaaggc tgtaggaaag gacaacttca ccctgattcc ggagggcacc aatggcactg
1141aggagcggat gtccgtcatc tgggacaagg ctgtggtcac tgggaagatg gatgagaacc
1201agtttgtggc tgtgaccagc accaatgcag ccaaagtctt caacctttac ccccggaaag
1261gccgcattgc tgtgggatcc gatgccgacc tggtcatctg ggaccccgac agcgttaaaa
1321ccatctctgc caagacacac aacagctctc tcgagtacaa catctttgaa ggcatggagt
1381gccgcggctc cccactggtg gtcatcagcc aggggaagat tgtcctggag gacggcaccc
1441tgcatgtcac cgaaggctct ggacgctaca ttccccggaa gcccttccct gattttgttt
1501acaagcgtat caaggcaagg agcaggctgg ctgagctgag aggggttcct cgtggcctgt
1561atgacggacc tgtgtgtgaa gtgtctgtga cgcccaagac agtcactcca gcctcctcgg
1621ccaagacgtc tcctgccaag cagcaggccc cacctgtccg gaacctgcac cagtctggat
1681tcagtttgtc tggtgctcag attgatgaca acattccccg ccgcaccacc cagcgtatcg
1741tggcgccccc cggtggccgt gccaacatca ccagcctggg ctacccaact ttcttgtaca
1801aagtggttgg taagcctatc cctaaccctc tcctcggtct cgattctacg tagtaatgag
1861ctagcgctaa ccggtggcgc gttaagtcga caatcaacct ctggatta

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