Construct: ORF ccsbBroad304_00463
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016420.1_s304c1, BRDN0000411522
- Derived from:
- ccsbBroadEn_00463
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DRD2 (1813)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_00463
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1813 | DRD2 | dopamine receptor D2 | NM_000795.4 | 100% | 100% | |
| 2 | human | 1813 | DRD2 | dopamine receptor D2 | XM_017017296.2 | 100% | 100% | |
| 3 | human | 1813 | DRD2 | dopamine receptor D2 | NM_016574.3 | 93.4% | 93.4% | 722_723ins87 |
| 4 | mouse | 13489 | Drd2 | dopamine receptor D2 | NM_010077.2 | 90.6% | 95.4% | (many diffs) |
| 5 | mouse | 13489 | Drd2 | dopamine receptor D2 | XM_006509996.3 | 84.3% | 89.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1395
- ORF length:
- 1329
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgga tccactgaat ctgtcctggt atgatgatga tctggagagg cagaactgga 121 gccggccctt caacgggtca gacgggaagg cggacagacc ccactacaac tactatgcca 181 cactgctcac cctgctcatc gctgtcatcg tcttcggcaa cgtgctggtg tgcatggctg 241 tgtcccgcga gaaggcgctg cagaccacca ccaactacct gatcgtcagc ctcgcagtgg 301 ccgacctcct cgtcgccaca ctggtcatgc cctgggttgt ctacctggag gtggtaggtg 361 agtggaaatt cagcaggatt cactgtgaca tcttcgtcac tctggacgtc atgatgtgca 421 cggcgagcat cctgaacttg tgtgccatca gcatcgacag gtacacagct gtggccatgc 481 ccatgctgta caatacgcgc tacagctcca agcgccgggt caccgtcatg atctccatcg 541 tctgggtcct gtccttcacc atctcctgcc cactcctctt cggactcaat aacgcagacc 601 agaacgagtg catcattgcc aacccggcct tcgtggtcta ctcctccatc gtctccttct 661 acgtgccctt cattgtcacc ctgctggtct acatcaagat ctacattgtc ctccgcagac 721 gccgcaagcg agtcaacacc aaacgcagca gccgagcttt cagggcccac ctgagggctc 781 cactaaaggg caactgtact caccccgagg acatgaaact ctgcaccgtt atcatgaagt 841 ctaatgggag tttcccagtg aacaggcgga gagtggaggc tgcccggcga gcccaggagc 901 tggagatgga gatgctctcc agcaccagcc cacccgagag gacccggtac agccccatcc 961 cacccagcca ccaccagctg actctccccg acccgtccca ccatggtctc cacagcactc 1021 ccgacagccc cgccaaacca gagaagaatg ggcatgccaa agaccacccc aagattgcca 1081 agatctttga gatccagacc atgcccaatg gcaaaacccg gacctccctc aagaccatga 1141 gccgtaggaa gctctcccag cagaaggaga agaaagccac tcagatgctc gccattgttc 1201 tcggcgtgtt catcatctgc tggctgccct tcttcatcac acacatcctg aacatacact 1261 gtgactgcaa catcccgcct gtcctgtaca gcgccttcac gtggctgggc tatgtcaaca 1321 gcgccgtgaa ccccatcatc tacaccacct tcaacattga gttccgcaag gccttcctga 1381 agatcctcca ctgctgccca actttcttgt acaaagtggt tggtaagcct atccctaacc 1441 ctctcctcgg tctcgattct acgtagtaat gagctagcgc taaccggtgg cgcgttaagt 1501 cgacaatcaa cctctggatt a